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Screening of Potential Antibiotic Action of Cellulolytic Fungi

机译：纤维素分解真菌潜在抗生素作用的筛选

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Twenty different strains of filamentous fungi were initially selected for evaluation of cellulolytic activity using a single test in a simple mineral salts culture medium with filter paper as the only carbon source. Those fungi strains that were capable of completely breaking the filter paper strip within 4-8 d were assayed also for antimicrobial action, using Staphyloccocus aureus ATCC 6538P according to the so-called agar piece method. We screened three different strains with both capacities: the production of cellulolytic activity and antibiotic action. The experimental results suggest that the fungi Penicillium sp. F0PC01, Aspergillus sp. F0Q001, and Cephalosporium sp. F03800 have both capabilities because they grew rapidly on cellulose as the only carbon source and were able to produce an area of growth inhibition in S. aureus of approx 2.04, 1.57, and 2.39 cm, respectively, on agar plates using the agar piece method. Subsequently, the antibiotic production obtained with those cellulolytic strains was evaluated by submerged fermentation at the flask level, in a simple culture medium containing lactose without biosynthesis precursor, obtaining 3670, 2830, and 4060 antibiotic units/mL, referred to as penicillin G, whereas for cellulolytic activity, the results were 1.34, 1.81 and 0.57 FPU/mL, respectively.
机译：首先在简单的无机盐培养基中以滤纸作为唯一碳源，通过一次测试选择了二十种不同的丝状真菌菌株，以评估纤维素分解活性。根据所谓的琼脂片法，使用金黄色葡萄球菌ATCC 6538P，还测定了能够在4-8天内完全破坏滤纸条的那些真菌菌株的抗微生物作用。我们筛选了具有两种能力的三种不同菌株：纤维素分解活性的产生和抗生素的作用。实验结果表明该真菌为青霉菌。 F0PC01，曲霉菌。 F0Q001和Cephalosporium sp。 F03800具有这两种功能，因为它们在琼脂平板上使用琼脂片法在纤维素上作为唯一的碳源迅速生长，并且能够在琼脂平板上分别产生约2.04、1.57和2.39 cm的金黄色葡萄球菌生长抑制面积。随后，通过在烧瓶级的深层发酵，在不含生物合成前体的含乳糖的简单培养基中，通过烧瓶中的水下发酵来评估由这些纤维素分解菌株获得的抗生素产量，从而获得3670、2830和4060抗生素单位/ mL，称为青霉素G。对于纤维素分解活性，结果分别为1.34、1.81和0.57 FPU / mL。

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