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A Stochastic Approach to Count RNA Molecules Using DNA Sequencing Methods

机译：使用DNA测序方法对RNA分子进行计数的随机方法

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Estimating the number of congeneric mRNA molecules in a preparation is an essential task in DNA technology and biochemistry. However, DNA sequencing methods can only distinguish molecules with different sequences or of different lengths. Recently, it was shown that it is possible to combine RNA molecules with short DNA molecules (tags) such that, with high probability, any two RNA molecules are combined with tags having different sequences. For this technique, we propose a statistical estimator and a confidence interval to determine the number of mRNA molecules in a preparation. In a second approach, the mRNA molecules are lengthened by attaching a random number of linker oligonucleotides. The original number of mRNA molecules is then determined by the number of different lengths obtained from the experiment. We also give estimator and confidence interval for this method. Both methods can be implemented using recent as well as established methods from DNA technology. The methods can also be applied to a larger number of molecules without the need to exhaust the complete preparation. The computation of estimators and confidence intervals can be accomplished by dynamic programming.
机译：估计制剂中同类mRNA的数量是DNA技术和生物化学中的基本任务。但是，DNA测序方法只能区分具有不同序列或不同长度的分子。近来，已表明可以将RNA分子与短DNA分子（标签）结合，使得很有可能将任意两个RNA分子与具有不同序列的标签结合。对于这项技术，我们提出了一个统计估计量和一个置信区间，以确定制剂中mRNA分子的数量。在第二种方法中，通过连接随机数的接头寡核苷酸来延长mRNA分子。然后通过从实验中获得的不同长度的数量来确定mRNA分子的原始数量。我们还给出了该方法的估计量和置信区间。两种方法都可以使用DNA技术的最新方法和已建立的方法来实施。该方法还可以应用于大量分子，而无需用尽整个制备过程。估计量和置信区间的计算可以通过动态编程来完成。

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