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Study on Submerged-Culture Condition ofFlammulina Velutipesliquid Strain in theFermentation Cylinder

机译:发酵缸中金针菇液体菌株的浸没培养条件研究

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The biological characters of deep culture is researched on Flammulina velutipes 908liquid strain in the fermentation cylinder. It’s determined that the suitable cultivated condition isculture temperature 24~25°C, pH5.5~7.0, inoculum concentration 15%. In the 24h, VVM1.0, thestirrer rotation speed 180r/min, within the time of 24~60h, VVM1.2, the rotate speed of cradle200r/min.The logarithimic growth phase of strain is limited in 24~60h. The experimental result is determined:the mycelial numbers 720/mL, contents of reducing sugar 5.96g/100mL, amino nitrogen9.54mg/100mL. enzymatic activity of cellulase 1.85u, starch 2.38u, laccase 0.092u, proteinase0.184u on end of logarithimic growth phase.
机译:金针菇908研究了深层培养的生物学特性 发酵缸中的液体应变。确定合适的栽培条件是 培养温度24〜25℃,pH5.5〜7.0,接种浓度15%。在24小时内,VVM1.0 搅拌器转速180r / min,在24〜60h的时间内,VVM1.2,支架的转速 200r /分钟 菌株的对数生长期限制在24〜60h。实验结果确定: 菌丝数720 / mL,还原糖含量5.96g / 100mL,氨基氮 9.54毫克/ 100毫升。纤维素酶1.85u,淀粉2.38u,漆酶0.092u,蛋白酶的酶活性 对数生长期结束时为0.184u。

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