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Effect of Formaldehyde on Cellular Proliferation of HEK293 cells

机译:甲醛对HEK293细胞增殖的影响

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In order to investigate the influences of formaldehyde with different doses on the cellular proliferation and cell cycle of the HEK293 cells, methyl thiazol tetrazolium (MTT) assay and flow cytometry were applied to measure the cellular proliferation and cell cycle. After the HEK293 cells were treated by low dose of formaldehyde (60μmol/L) for 12 hours, the percentage of phase G1 (28.4%) was decreased 23.24% compared with that of the control group (37.0%), but the percentage of phase S (48.5%)was increased 19.16% compared with that of the control group (40.7%); After the HEK293 cells were treated by the high dose of formaldehyde (240μmol/L) for 12 hours, the percentage of phase G1 (39.9%) was increased 7.8% compared with that of the control group (37.0%), the percentage of phase S (32.8%) was decreased 19.41% compared with that of the control group (40.7%). The results demonstrate that formaldehyde can promote the cellular proliferation of the HEK293 cells at the low dose (60μmol/L) and can inhibit the cellular proliferation of the HEK293 cells at the high dose (240 μmol/L).
机译:为了研究不同剂量甲醛对HEK293细胞增殖和细胞周期的影响,应用甲基噻唑四唑(MTT)法和流式细胞仪检测了甲醛对HEK293细胞的增殖和细胞周期的影响。用低剂量甲醛(60μmol/ L)处理HEK293细胞12小时后,G1期的比例(28.4%)比对照组(37.0%)降低了23.24%,但相的百分比S(48.5%)比对照组(40.7%)增加了19.16%; HEK293细胞用高剂量甲醛(240μmol/ L)处理12小时后,G1期的百分比(39.9%)比对照组(37.0%)增加了7.8%。 S(32.8%)与对照组(40.7%)相比降低了19.41%。结果表明,甲醛可以在低剂量(60μmol/ L)时促进HEK293细胞的细胞增殖,而在高剂量(240μmol/ L)时可以抑制HEK293细胞的细胞增殖。

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