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首页> 外文会议>Silicones and silicone-modified materials symposium >Chapter 21 The Interaction of 'Silicon' with Proteins: Part 2. The Rold of Bioinspired Peptide and Recombinant Proteins in Silica Polymerization
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Chapter 21 The Interaction of 'Silicon' with Proteins: Part 2. The Rold of Bioinspired Peptide and Recombinant Proteins in Silica Polymerization

机译：第21章“硅”与蛋白质的相互作用：第2部分。生物启发的肽和重组蛋白质在二氧化硅聚合中的作用

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Various organisms are known to produce hierarchically organised ornate biosilica structures in vivo with great fidelity. In several recent studies it has been proposed that the controlled moulding of biosilicas is regulated by biomolecules such as peptides, proteins and polysaccharides. However, the mechanisms underpinning biomolecule mediated biosilici-fication and the interactions of 'silicon' with biomolecules remain unclear. The aim of this paper is to carry out model studies of silicification with the intention of probing the interactions between biological molecules derived from biosilicifying organisms with silica. We present a detailed study of silica formation in the presence of a recombinant silicatein protein (derived from the sponge Suberites domuncula) and an R5 peptide (derived from silaffin proteins isolated from the diatom Cylindrotheca fusiformis). We have also undertaken model studies of silica formation using recombinant proteins of known primary and secondary structure and pI to investigate the effect that structure has on structure development from solution. A silicon-catecholate complex was used that liberates silicic acid under circumneutral pH. The kinetics of silicic acid polymerisation was monitored using a colorimetric method. The aggregation of polysilicic acid oligomers and particle growth was studied using dynamic light scattering. The nature of the solid materials formed was investigated by electron microscopy -transmission electron microscopy to study the sizes of the fundamental particles and scanning electron microscopy to study the morphology of aggregates. In order to assess surface area, porosity and pore size distributions, gas adsorption analysis was performed. Thermal analysis was used to quantify the amount of organic material entrapped into silicas, which may originate from the catechol released after decomposition of the silicon complex or from the protein or peptide added to the reaction mixture. It was found that these functional additives affect all aspects of silicic acid polymerisation. In particular, the aforesaid proteins/peptides were able to control the kinetics of silica polymerisation. In addition, most of these additives modulated the surface area and pore size distribution of the silicas prepared in their presence.
机译：已知各种生物可以在体内高度保真地产生分层组织的华丽生物二氧化硅结构。在最近的一些研究中，已经提出生物硅的受控模制是由诸如肽，蛋白质和多糖的生物分子来调节的。然而，支撑生物分子介导的生物硅化的机制以及“硅”与生物分子的相互作用仍然不清楚。本文的目的是进行硅化的模型研究，以探究生物硅化生物衍生的生物分子与二氧化硅之间的相互作用。我们提出了在重组硅酸盐蛋白（源自海绵Suberites domuncula）和R5肽（源自硅藻蛋白fusiformis的silaffin蛋白）存在下二氧化硅形成的详细研究。我们还使用已知的一级和二级结构以及pI的重组蛋白进行了二氧化硅形成的模型研究，以研究结构对溶液中结构发展的影响。使用了儿茶酚酸硅络合物，该络合物在环境pH值下释放出硅酸。使用比色法监测硅酸聚合的动力学。使用动态光散射研究了聚硅酸低聚物的聚集和颗粒的生长。通过电子显微镜-透射电子显微镜研究基本颗粒的尺寸，并通过扫描电子显微镜研究聚集体的形态来研究形成的固体材料的性质。为了评估表面积，孔隙率和孔径分布，进行了气体吸附分析。使用热分析来定量分析截留在二氧化硅中的有机物的量，这可能是由于硅配合物分解后释放出的邻苯二酚或源自添加到反应混合物中的蛋白质或肽。发现这些功能性添加剂影响硅酸聚合的所有方面。特别地，上述蛋白质/肽能够控制二氧化硅聚合的动力学。另外，大多数这些添加剂调节了在它们的存在下制备的二氧化硅的表面积和孔径分布。

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