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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >Effect of osmopriming on germination in seeds of three winter canola (Brassica napus L.)varieties under salinity stress
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Effect of osmopriming on germination in seeds of three winter canola (Brassica napus L.)varieties under salinity stress

机译：渗透胁迫对盐分胁迫下三种冬季油菜（Canass napus L.）品种种子萌发的影响

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Effects of salinity (in four levels) and priming (in eleven levels with control) on germination and growth parameters of three canola varieties (SLM046, Okapi and Licord) were investigated under laboratory (first experiment) and greenhouse (second experiment) conditions with three replications. The statistical design was factorial laid out in complete randomized design and randomized complete block design in the first and second experiment respectively. At first, seeds were primed in two times (12 and 24 hours) and five osmotic potentials by polyethylenglycol (-10, -12, -14, -16 and -18 bar) in petridishes. At the end of priming, seeds were washed by distilled water, and used for germination test in laboratory and greenhouse conditions.Germination tests of control and primed seeds were conducted in four levels of salinity (0, 6, 12 and 18 ds/m). Aft er planting the seeds, counting germinated seeds was done daily for one week in the first experiment and two weeks in the second one and germination percentage and index and coefficient of velocity were calculated. Other parameters, include length and dry weight of stem and radicle (in first examination) and leaf area, chlorophyll content, plant dry weight, leaf area and leaf weight ratio, specific leaf area and vigour index (in second examination) were measured. Salinity reduced germination percentage and index, coefficient of velocity and vigour index at early stages of canola growth, at normal and salinity stress in laboratory and greenhouse conditions.In laboratory condition, salinity at the level of 6 ds/m increased stem, radicle and seedling dry weight, stem and radicle length, but these parameters were reduced by higher level of salinity, and they became lower than control treatment. Also in greenhouse condition, salinity deceased plant dry weight, leaf area, chlorophyll content, leaf area and weight ratio and specific leaf area. In laboratory conditions priming in 24 hours increased germination percentage, but this parameter was decreased by 12 hours priming. Priming treatment reduced germination index, stem, radicle and seedling dry weight, stem length and vigour index and increased coefficient of velocity and radicle length in compare with control. In greenhouse conditions, priming increased the coefficient of velocity, chlorophyll content, leaf area, leaf area and leaf weight ratio (in 24 hours priming) and specific leaf area,and decreased emergence percentage and index, leaf weight ratio (in 12 hours priming) and vigour index.
机译：在实验室（第一个实验）和温室（第二个实验）条件下，研究了盐度（四个级别）和启动（十一个对照级别）对三种油菜品种（SLM046，Okapi和Licord）的萌发和生长参数的影响。复制。在第一个和第二个实验中，统计设计分别在完全随机设计和随机完整块设计中进行了因子设计。首先，在peridishes中用聚乙烯乙二醇（-10，-12，-14，-16和-18 bar）在两次（12和24小时）和五种渗透势中引发种子。底涂结束后，用蒸馏水洗涤种子，并在实验室和温室条件下用于发芽测试。对照和底涂种子的发芽测试在四个盐度水平（0、6、12和18 ds / m）下进行。在种植种子后，在第一个实验中每天进行发芽种子计数，每天进行一次，在第二个实验中进行两周，计算出发芽率，指数和速度系数。其他参数包括茎和胚根的长度和干重（在第一次检查中）和叶面积，叶绿素含量，植物干重，叶面积和叶重量比，比叶面积和活力指数（在第二次检查中）。在实验室和温室条件下，盐分降低了油菜生长初期，正常和盐分胁迫下油菜籽生长初期的发芽率和指数，速度系数和活力指数。在实验室条件下，盐分在6 ds / m时增加了茎，胚根和幼苗的发芽率干重，茎和胚根长度，但这些参数因盐度较高而降低，并且比对照处理低。同样在温室条件下，盐度降低了植物的干重，叶面积，叶绿素含量，叶面积和重量比以及比叶面积。在实验室条件下，在24小时内启动会增加发芽率，但在12小时内启动会降低该参数。与对照相比，引物处理降低了发芽指数，茎，胚根和幼苗的干重，茎长度和活力指数，并增加了速度和胚根长度的系数。在温室条件下，引发增加了速度系数，叶绿素含量，叶面积，叶面积和叶重量比（引发24小时）和比叶面积，并且降低了出苗率和指数，叶片重量比（引发12小时）和活力指数。

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