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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >'THE LATEST IN SEED COAT FASHION': seed colour (proanthocyanidin)and trichome mutations in a new population of activation-tagged Arabidopsis lines
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'THE LATEST IN SEED COAT FASHION': seed colour (proanthocyanidin)and trichome mutations in a new population of activation-tagged Arabidopsis lines

机译：'最新的种子外套时尚'：带有激活标签的拟南芥品系新种群中的种子颜色（原花色素）和毛状体突变

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Flavonoids comprise a diverse group of phenolic compounds, which serve a variety of ecological and physiological functions in plants. These include attraction of pollinators, defense against predators and pathogens, and protection against UV damage. Flavonoid polymers (proanthocyanidins, also known as condensed tannins) are advantageous for controlling ruminant digestion and insect foraging. They also have wide-ranging benefits in human health. In the oilseed and vegetable Brassicas, they are present inbrown and black seed coats as a part of the anti-nutritional phenolic fibre fraction and their reduction is the focus of major breeding programmes around the world. Several collections of chemically-induced and T-DNA-induced mutants in the Arabidopsis flavonoid pathway have been screened worldwide to find transparent testa (tt) and tannin-deficient seed (tds) mutants in the proanthocyanidin pathway. These studies have led to a detailed understanding of the enzymology and metabolic regulation of much of the flavonoid pathway. Seventeen flavonoid genes have been characterized over the past decade in Arabidopsis,including six regulatory genes, a vacuole transporter, and eleven structural genes, but genes defining the last steps in polymer formation are unknown. Recently, a new population of activation-tagged Arabidopsis lines (～50,000) and T-DNA -tagged lines (～20,000) were developed in Saskatoon, Canada. Within this population, we have discovered additional seed coat colour alleles,interacting trichome alleles, and several novel proanthocyanidin-free mutants. The lines are being used to re-define the pathway and as a basis for isolating novel genes.
机译：类黄酮包含多种酚类化合物，它们在植物中具有多种生态和生理功能。这些措施包括吸引传粉媒介，防御捕食者和病原体以及保护免受紫外线伤害。类黄酮聚合物（原花色素，也称为缩合单宁）有利于控制反刍动物的消化和昆虫的觅食。它们在人类健康方面也具有广泛的益处。在油菜籽和蔬菜芸苔中，它们存在于棕色和黑色种皮中，作为抗营养酚醛纤维成分的一部分，其减少是全球主要育种计划的重点。全世界已经筛选了拟南芥类黄酮途径中化学诱导的和T-DNA诱导的突变体的几个集合，以在原花青素途径中找到透明的睾丸（tt）和单宁缺乏的种子（tds）突变体。这些研究导致对许多类黄酮途径的酶学和代谢调控的详细了解。在过去的十年中，已经在拟南芥中鉴定了17个类黄酮基因，包括6个调节基因，液泡转运蛋白和11个结构基因，但是定义聚合物形成最后步骤的基因尚不清楚。最近，在加拿大萨斯卡通开发了一批新的带有激活标记的拟南芥品系（〜50,000）和带有T-DNA标记的品系（〜20,000）。在这一种群中，我们发现了其他种皮颜色等位基因，相互作用的毛状体等位基因，以及一些新的无原花色素的突变体。该品系被用于重新定义该途径，并作为分离新基因的基础。

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