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The establishment of high frequency somatic embryogenesis and plant regeneration system of Eruca sativa Mill

机译:苜蓿高频体细胞胚发生和植株再生体系的建立。

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A protocol was developed for high frequency somatic embryogenesis and plant regeneration of E. sativa Cotyledon explants grown on MS medium supplemented with 1.0mg/L 2,4-D formed embryogenic callus after 4 weeks culture. Secondary somatic embryos were also produced from that on MS medium containing 0.2mg/L2, 4-D. Somatic embryos differentiated into mature embryos on N6 medium. Mature somatic embryos developed into plantlets by culturing the mature somatic embryos on 1/2MS medium containing 0.1mg/L IBA.
机译:开发了协议,用于高频体细胞胚发生和植物再生4周后,在补充有1.0mg / L 2,4-D形成的胚性愈伤组织的MS培养基上生长的苜蓿子叶外植体的高频体细胞胚发生和植物再生。还从含有0.2mg / L2,4-D的MS培养基上产生了次级的体细胞胚。体细胞胚在N6培养基上分化为成熟胚。通过在含有0.1mg / L IBA的1 / 2MS培养基上培养成熟的体细胞胚,可将成熟的体细胞胚发育为小植株。

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