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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >Terminal-Repeat Retrotransposon In Miniature (TRIM) elements and their potential utility as DNA markers in Brassica relatives
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Terminal-Repeat Retrotransposon In Miniature (TRIM) elements and their potential utility as DNA markers in Brassica relatives

机译：终末微型反转录转座子（TRIM）及其在芸苔属中的潜在DNA标记作用

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摘要

Based on genome wide search for identification of Terminal-Repeat Retrotransposon In Miniature (TRIM) elements, we characterized eight TRIM families; four from Brassica and four from Arabidopsis (including three families which were identified before) in the family Brassicaceae. The elements contain 100～257 bp terminal repeats (TR) and 100～800 bp internal sequences.Studies of insertion site polymorphisms of each element across taxa infer the taxonomic lineage in the family Brassicaceae and the insertion time of each element. TRIM display experiments revealed abundant polymorphic bands among taxa in Brassicaceae and among commercial F1 varieties of Chinese cabbage and radish suggesting the potential utility of TRIM elements as DNA markers for the study of genome evolution, genome mapping, and variety protection for any Brassica crops. Other TRIM features that are advantageous for such studies include their miniature size, insertion preference in euchromatin regions, and ubiquitous distribution as evident in the Arabidopsis genome.
机译：基于全基因组搜索以识别微型末端重复序列反转录转座子（TRIM），我们鉴定了8​​个TRIM家族。芸苔科中的四个来自芸苔属，四个来自拟南芥（包括三个以前鉴定的科）。这些元件包含100〜257 bp的末端重复序列（TR）和100〜800 bp的内部序列。研究每个单元在类群中的插入位点多态性可以推断十字花科的分类谱系和每个单元的插入时间。 TRIM展示实验揭示了十字花科的类群之间以及大白菜和萝卜的商业F1品种之间存在丰富的多态性条带，这表明TRIM元素作为DNA标记物的潜在用途，可用于研究任何芸苔属作物的基因组进化，基因组图谱和品种保护。对于此类研究有利的其他TRIM功能包括其微型尺寸，在常染色质区域的插入偏好以及在拟南芥基因组中显而易见的普遍分布。

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