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首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >Identification of the corresponding avirulence gene of Leptosphaeria maculans to the resistance gene (LepR1) in Brassica napus
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Identification of the corresponding avirulence gene of Leptosphaeria maculans to the resistance gene (LepR1) in Brassica napus

机译：芸苔油菜对斑节菜的抗性基因（LepR1）的相应无毒力基因的鉴定

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Blackleg of canola is the most important disease of canola/rapeseed worldwide. Efforts are made to understand the host-pathogen interaction by studying the interaction between resistance genes of the host and the avirulence genes of the pathogen. Previous studies showed that two double haploid canola (Brassica napus L.) lines, ddm-12-65-1 and ddm-12-65-2, possess a novel resistance gene (LepR1) against PGT (99-43 & 99-56) and PG2 (WA-74) isolates. However, these two lines are susceptible to PG2 isolate 87-41. It is thought that the avirulence of isolates WA-74, 99-43 and 99-56 on the lines ddm-12-65-1 and ddm- 12 -65-2 is governed by the Avr1/LepR1 and the virulence of isolate 87 -41 is governed by avr1/LepR1. Isolates 87-41 and 99-56 were selected as virulent and avirulent isolates based on phenotypic reactions caused by the isolates on the lines and their mating type. They were crossed and the progenies segregated based on the phenotypic reaction on the line ddm- 12-6-1. The 87-41 × 99-56 L. maculans map will be constructed using PCR-based markers including single sequence repeats (SSR) and sequence related amplified polymorphic (SRAP) markers. Polymorphic bands will be recorded as present or absent in the parents and in the whole progeny. Once the physical distances of the Avr gene with its markers are identified, the genomic BAC library from L.maculans isolate 99-56 will be constructed. The library will be screened using the closely linked markers and a combination of markers could then be used to identify positive clones.
机译：双低油菜籽的黑腿病是全球双低油菜籽/油菜籽的最重要疾病。通过研究宿主的抗性基因和病原体的无毒力基因之间的相互作用，努力了解宿主-病原体的相互作用。先前的研究表明，两个双单倍油菜油菜（dds-12-65-1和ddm-12-65-2）拥有对PGT的新型抗性基因（LepR1）（99-43和99-56） ）和PG2（WA-74）分离株。但是，这两条线易受PG2分离株87-41的影响。据认为，ddm-12-65-1和ddm-12 -65-2品系的WA-74、99-43和99-56分离株的无毒性受Avr1 / LepR1和分离株87的毒力控制。 -41由avr1 / LepR1控制。根据分离株系及其交配类型引起的表型反应，将分离株87-41和99-56选为有毒和无毒分离株。将它们杂交并基于ddm-12-6-1系的表型反应分离后代。将使用基于PCR的标记（包括单序列重复（SSR）和与序列相关的扩增多态性（SRAP）标记）构建87-41×99-56的黄斑狼疮图。多态性条带将在亲本和整个后代中记录为存在或不存在。一旦鉴定了Avr基因及其标记的物理距离，就将构建来自L.maculans分离株99-56的基因组BAC文库。将使用紧密连接的标记物筛选文库，然后可以使用标记物组合来鉴定阳性克隆。

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