首页> 外文会议>Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production >A preliminary study on glucosinolates heterosis in leaf of hybrids in Brassica napus L
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A preliminary study on glucosinolates heterosis in leaf of hybrids in Brassica napus L

机译:甘蓝型油菜杂交种叶片硫代芥子油苷杂种优势的初步研究

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Brassica napus L. is widely grown in Yangzi River valley of China as a major edible oilseed. Because of the unpleasant smell and toxicity from glucosinolates degradation products, genetic improvement has been made to decrease glucosinolates concentration in seed by breeding. However, it was also found that leaf glucosinolates concentration and profiles were closely related to plant diseases resistance and the taste of shoot as vegetable. And anticancer effects from degradation products of indolyl and other glucosinolates were also reported. Recently, new varieties of Brassica napus L. used both for vegetable and oilseeds were released and popularized in the middle and lower valley of Yangzi River. Rapeseed with high glucosinolate content in leaves and low content in seeds were considered as ideal for both seed quality and plant diseases resistance. Therefore leaf glucosinolatescall for more and more attention. In this paper the variation of leaf glucosinolates content in all growth periods and the heterosis of glucosinolates in leaf were studied with hybrids and their parents as well as the double high material by high performance liquid chromatography (HPLC). The results showed that Leaf glucosinolate content in double-high material Zhongyou 821 decreased from rosette to blooming stage, while in double-low material leaf glucosinolate increased from overwintering to budding period,decreased at stem elongation stage and rose again at early blooming stage. Leaf glucosinolate contents and profiles varied significantly in double-high and double-low rapeseeds although aliphatic glucosinolates were the dominant ones in both leaves.All crosses presented mainly negative heterosis over both parents. The heterosis of leaf glucosinolates of F1 and F2 hybrids were significant and negative in stem elongation and floral period, suggesting that it would be difficult to increase leaf glucosinolates in these periods for high disease resistance and it might be the best time for harvesting shoots as vegetable.
机译:甘蓝型油菜是中国主要的食用油籽,广泛种植于中国扬子江流域。由于芥子油苷降解产物具有令人不愉快的气味和毒性,因此已经进行了遗传改良以通过育种降低种子中芥子油苷的浓度。然而,还发现叶片中的芥子油苷的浓度和分布与植物抗病性和作为蔬菜的芽的味道密切相关。还报道了吲哚基和其他芥子油苷降解产物的抗癌作用。近来,用于菜籽和油料籽的甘蓝型油菜新品种在扬子江中下游流域广泛推广。叶中芥子油苷含量高而种子中含量低的菜籽被认为是种子品质和植物抗病性的理想选择。因此叶芥子油苷受到越来越多的关注。本文利用杂种及其亲本以及双重高效材料,通过高效液相色谱法(HPLC)研究了叶片在整个生长期中芥子油苷含量的变化和芥子油苷的杂种优势。结果表明,双高材料中油821的芥子油苷含量从莲座丛到开花期降低,而双低材料中的芥子油苷的含量从越冬到出芽期增加,在茎伸长期降低,在初花期又升高。双脂和双低油菜籽中的芥子油苷含量和谱型差异很大,尽管脂族芥子油苷是两叶中的优势种。所有杂交对两亲均表现出负的杂种优势。 F1和F2杂种叶片芥子油苷的杂种优势在茎伸长和花期中显着且为负值,这表明在这些时期很难增加叶片芥子油苷的抗病性,这可能是收获新芽作为蔬菜的最佳时机。 。

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