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首页> 外文会议>Symposium on Biotechnology for Fuels and Chemicals >Evaluation of a Hypocrea jecorina Enzyme Preparation for Hydrolysis of Tifton 85 Bermudagrass
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Evaluation of a Hypocrea jecorina Enzyme Preparation for Hydrolysis of Tifton 85 Bermudagrass

机译：水解Tifton 85百慕大草的红景天酶制剂的评价

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Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techniques. A previously developed pressurized batch hot water (PBHW) method was used to treat Tifton 85 bermudagrass for enzymatic hydrolysis. Native grass (untreated) and PBHW-pretreated material were compared as substrates for fungal cultivation to produce enzymes. Cellulase activity, measured via the filter paper assay, was higher for fungi cultivated on PBHW-pretreated grass, whereas the other nine enzyme assays produced higher activities for the untreated grass. Ferulic acid and vanillin levels increased significantly for the enzyme preparations produced using PBHW-pretreated grass and the release of these phenolic compounds may have contributed to the observed reduction in enzyme activities. Culture supernatant from Tifton 85 bermudagrass-grown fungi were combined with two commercial enzyme preparations and the enzyme activity profiles are reported. The amount of reducing sugar liberated by the enzyme mixture from Hypocrea jecorina (after 192 h incubation with untreated bermudagrass) individually or in combination with feruloyl esterase was 72.1 and 84.8%, respectively, of the commercial cellulase preparation analyzed under the same conditions.
机译：在佐治亚州蒂夫顿的ARS-USDA上开发的Tifton 85百慕大草，在美国超过一千万英亩的干草和草料种植地上生长。在百慕大草品种中，Tifton 85具有更好的消化率，因为使用传统的植物育种技术已经降低了醚键与酯键连接的酚酸的比例。先前开发的加压分批热水（PBHW）方法用于处理Tifton 85百慕大草以进行酶促水解。比较了天然草（未经处理）和经PBHW预处理的材料作为真菌培养产生酶的底物。通过PBWW预处理的草种植的真菌，通过滤纸分析测得的纤维素酶活性更高，而其他九种酶的分析对未处理的草则表现出更高的活性。使用PBHW预处理的草生产的酶制剂中的阿魏酸和香兰素水平显着增加，这些酚类化合物的释放可能导致观察到的酶活性降低。来自Tifton 85百慕大草生长真菌的培养上清液与两种市售酶制剂结合，并报道了酶活性图谱。在相同条件下分析的商业纤维素酶制剂中，从红褐肉座菌（与未经处理的百慕大草孵育192小时后）的酶混合物释放的还原糖量分别为分别为72.1和84.8％。

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