Suparmolecular vesicular aggregates (SVAs) wereachieved by self-assembling of an ,on-poly(aspartylhydrazide) copolymer bearing both folatemoieties and lipid anchors with Dipalmitoylphosphatidyl-choline and cholesterol, thus forming avesicular structure with the polymeric chains on thesurface. The physicochemical and pharmaceuticalparameters of SAVs were investigated. Gemcitabine wasused as a drug model. The in vitro anticancer activity ofSVAs was evaluated against breast cancer cells (MCF-7and BxPC-3 cell lines). Confocal laser scanningmicrosopy and flow cytometric analysis, as well as invitro binding experiments, were carried out to evaluatethe folate targeting features and the intracellular druguptake. Pharmacokinetic and biodistribution experimentswere also carried out.
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