• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009 >Artificial Antigen Synthesis and Antibody Production for Di(2-Ethylhexyl) Phthalate
【24h】

Artificial Antigen Synthesis and Antibody Production for Di(2-Ethylhexyl) Phthalate

机译：邻苯二甲酸二（2-乙基己基）酯的人工合成与抗体制备

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A hapten di(2-ethylhexyl) 4-aminophthalate (DEHAP) which possesses the structural feature of di(2-ethylhexyl) phthalate (DEHP) was synthesized via esterification and reduction with 4-nitrophthalic acid and 2-ethyl-1-hexanol. The product was confirmed by infrared absorption spectroscopy (IR), 1H nuclear magnetic resonance spectroscopy (1H NMR) and mass spectrometry (MS). Then glutaraldehyde was used as crosslinker to link DEHAP and bovine serum albumin (BSA) to make artificial immunogen DEHAP-BSA. DEHAP was linked to ovalbumin (OVA) through diazotization to prepare coating antigen DEHAP-OVA. The structure of artificial antigens was identified by ultraviolet spectroscopy (UV) and SDS polyacrylamide gels electrophoresis (SDS-PAGE). BALB/c mice were immunized with DEHAP-BSA and the titer of antisera they produced were detected by indirect enzyme-linked immunosorbent assay (ELISA). Results showed that the artificial antigens for DEHP were synthesized successfully and specific polyclonal antibodies with high titer were produced, which made it possible to obtain monoclonal antibodies and establish immunoassay for DEHP.
机译：具有邻苯二甲酸二（2-乙基己基）酯（DEHP）结构特征的半抗原二（2-乙基己基）4-氨基邻苯二甲酸酯（DEHAP）是通过4-硝基邻苯二甲酸和2-乙基-1-己醇的酯化和还原反应合成的。通过红外吸收光谱（IR）， 1 H核磁共振光谱（ 1 H NMR）和质谱（MS）确认了该产物。然后以戊二醛为交联剂将DEHAP和牛血清白蛋白（BSA）连接起来，制成人工免疫原DEHAP-BSA。通过重氮化将DEHAP与卵清蛋白（OVA）连接，以制备包被抗原DEHAP-OVA。人工抗原的结构通过紫外光谱（UV）和SDS聚丙烯酰胺凝胶电泳（SDS-PAGE）进行鉴定。用DEHAP-BSA免疫BALB / c小鼠，并通过间接酶联免疫吸附测定（ELISA）检测它们产生的抗血清的效价。结果表明，成功合成了DEHP的人工抗原，并制备了高滴度的特异性多克隆抗体，从而有可能获得单克隆抗体并建立DEHP的免疫分析方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号