• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>The 5th International Conference on Computer Sciences and Convergence Information Technology >Detection of serum anti-p53 antibodies in cancer that uses recombined wild-type p53 protein and phage displayed peptide
【24h】

Detection of serum anti-p53 antibodies in cancer that uses recombined wild-type p53 protein and phage displayed peptide

机译：使用重组的野生型p53蛋白和噬菌体展示肽检测癌症中的血清抗p53抗体

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Although clinical significance of serum p53 Abs (s-p53 Abs) in cancer patients has been studied during the past few years, how to detect it effectively is a crucial issue. Methods: wild-type p53 protein (wt p53) and phage-displayed peptide were prepared. Three types of enzymelinked immunosorbent assays (ELISA) methods were constructed and a hybrid antigen-ELISA approach was attempted. Results: 7 breast cancer patients (19.4%) were detected s-p53 Abs positive by p53-ELISA, 4 patients (11.1%) were detected positive by phage-ELISA, and 10 patients (27.8%) were detected positive by p53-phage ELISA. A combination of 1.5μg/ml wt p53 and 40μg/ml phage was the best concentration for a mix antigen in hybrid antigen-ELISA. Conclusion: A combination of p53-ELISA and phage-ELISA can effectively improve the sensitivity of s-p53 Abs. This increases the application possibility of s-p53 Abs on clinic.
机译：背景：尽管在过去的几年中研究了血清p53 Abs（s-p53 Abs）在癌症患者中的临床意义，但如何有效检测它却是一个至关重要的问题。方法：制备野生型p53蛋白（wt p53）和噬菌体展示肽。构建了三种类型的酶联免疫吸附测定（ELISA）方法，并尝试了一种混合抗原-ELISA方法。结果：p53-ELISA检测到7例乳腺癌患者（19.4％）s-p53 Abs阳性，噬菌体ELISA检测到4例患者（11.1％），p53噬菌体检测到10例患者（27.8％）。 ELISA。在混合抗原ELISA中，混合抗原的最佳浓度为1.5μg/ ml wt p53和40μg/ ml噬菌体。结论：p53-ELISA和噬菌体-ELISA联用可有效提高s-p53抗体的敏感性。这增加了s-p53 Abs在临床上的应用可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号