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首页> 外文会议>32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society >Self-assembly and recrystallization of bacterial S-layer proteins of Bacillus sphaericus and Bacillus thuringiensis on silicone, mica and quartz crystal supports
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Self-assembly and recrystallization of bacterial S-layer proteins of Bacillus sphaericus and Bacillus thuringiensis on silicone, mica and quartz crystal supports

机译：球形芽孢杆菌和苏云金芽孢杆菌细菌S层蛋白在有机硅，云母和石英晶体载体上的自组装和重结晶

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摘要

The Oriented architecture of macromolecules plays a critical role in many aspects of Nanobiotechnology such as in the development of biosensors. To this regard, S-layers which constitute the outermost cell envelope component of many prokaryotic organisms, represent unique self assembled systems with the capability to rearrange into monomolecular and oriented arrays. These properties can be exploited to promote their crystallization on surfaces (e.g. silicone) which is pivotal for the subsequent immobilization of macromolecules and development of new biosensors. In this work the crystallization of bacterial S-layers obtained from Bacillus thuringiensis and Bacillus sphaericus CCM2177 on silicone, mica and quartz crystal surfaces were investigated. The SDS page results of S-layers isolated from the above mentioned bacteria put in evidence that their molecular weight (MW) was around 120 KDa and, as reported in the literature, slightly higher for those extracted by Bacillus thuringiensis. In addition, results showed that S-layers isolated from Bacillus thuringiensis form large crystalline domains on mica after 5 min whereas those extracted from Bacillus sphaericus CCM 2177 form a compact monolayer on silicone after 2 h. Results in this work put in evidence the possibility to use these substrates for the fabrication of sensitive biosensors.
机译：大分子的面向体系结构在纳米生物技术的许多方面，例如在生物传感器的开发中，都起着至关重要的作用。就这一点而言，构成许多原核生物最外层细胞包膜成分的S层代表了独特的自组装系统，能够重新排列成单分子和定向阵列。可以利用这些特性来促进它们在表面（例如硅树脂）上的结晶，这对于随后的大分子固定和新生物传感器的开发至关重要。在这项工作中，研究了从苏云金芽孢杆菌和球形芽孢杆菌CCM2177获得的细菌S层在硅酮，云母和石英晶体表面的结晶。从上述细菌中分离出的S层的SDS页面结果表明，其分子量（MW）约为120 KDa，并且如文献报道的那样，由苏云金芽孢杆菌提取的分子量略高。此外，结果表明，从苏云金芽胞杆菌分离出的S层在5分钟后在云母上形成大的晶域，而从球形芽孢杆菌CCM 2177提取的S层在2 h后在硅酮上形成了紧密的单层。这项工作的结果表明，有可能使用这些基板来制造敏感的生物传感器。

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