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首页> 外文会议>Conference on key technology of horticulture >Effect of Recovery Culture on in vitro Plantlet Growth of Rhubarb
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Effect of Recovery Culture on in vitro Plantlet Growth of Rhubarb

机译：恢复培养对大黄试管苗生长的影响

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Continuous subculture resulted in growth deterioration in rhubarb(Rheum rhaponticum) micropropagation. By removing plant growth regulator in culture medium, strong rhubarb plantlets were regenerated from explants of adventurous buds. The recovery culture medium was MS medium supplemented with sucrose at 30g·l-1, ager at 5.2 g·l-1. In the further, part of the morphological and physiological traits, such as plantlet height, leaf number per plantlet, leaf size, leaf colour, plantlet biomass, chlorophyll content, photosynthesis rate, stomatal index had been compared between the recovery plantlets and subculture plantlets.
机译：连续的继代培养导致大黄（Rheum rhaponticum）微繁殖的生长恶化。通过去除培养基中的植物生长调节剂，可以从冒险芽的外植体中再生出大黄苗。回收培养基为补充有蔗糖30g·l-1，老化剂5.2g·l-1的MS培养基。此外，还比较了恢复苗和继代培养苗的部分形态和生理特征，例如苗高，每株苗的叶数，叶大小，叶色，苗生物量，叶绿素含量，光合作用率，气孔指数。

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