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Tissue Culture and Rapid Propagation Research of a New Species Medicinal Plant Gynura Medica

机译:一种新种药用植物绞股蓝的组织培养和快速繁殖研究

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In order to establish the method of rapid propagation of Gynura Medica, explants derived from leaf and axillary bud of Gynura Medica were cultured on ten induction media containing different concentration combination of 6-BA and NAA to induce microshoot. Leaf callus induction rates of medium 3 is the highest, compared with the other nine media, up to 90.0%, while leaf callus hardly differentiate shoots. On all ten induction media, axillary bud can be reduced to form shoots with more than 40 shoots per axillary bud. Shoots induced from axillary bud were regenerated, and transferred to 6 rooting media. The rooting medium SGI is proper to induce shoots rooting. After hardening, shoots were transplanted, and survival rate is up to 98%. Gynura Medica can be rapid propagated through inducing microshoot from axillary bud to obtain a number of young plants.
机译:为了建立灵芝的快速繁殖方法,将灵芝叶片和腋芽的外植体培养在十种含有6-BA和NAA不同浓度组合的诱导培养基上,以诱导微芽。与其他9种培养基相比,培养基3的叶片愈伤组织诱导率最高,达90.0%,而叶片愈伤组织几乎不能分化芽。在所有十种诱导培养基上,腋芽可以减少形成芽,每个腋芽中可以有40多个芽。由腋芽诱导的芽被再生,并转移到6个生根培养基中。生根培养基SGI适合诱导芽生根。硬化后,将芽移植,成活率高达98%。可以通过诱导腋芽的微芽来快速繁殖Gynura Medica,从而获得许多幼株。

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