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首页> 外文会议>Conference on key technology of horticulture >Molecular Cloning of cDNA Encoding Xyloglucan Endotransglucosylase/Hydrolase (XTH) in Tagetes Patula Petals
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Molecular Cloning of cDNA Encoding Xyloglucan Endotransglucosylase/Hydrolase (XTH) in Tagetes Patula Petals

机译：CDNA CDNA编码Xyloglucan内甘油糖苷基酶/水解酶（Xth）的CDNA的分子克隆

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摘要

Xyloglucan endotransglucosylase/hydrolase (XTH) is a kind of cell wall relaxation factor and has already been confirmed to be related with cell extension, fruit softening, petal senescence and so on. In the present study, to discuss the structure features of the XTH genes in compositae, the petals of Tagetes Patula, which belongs to the ethylene-insensitive plant, was used as experiment materials. As a result, the cDNA of XTH gene with 906bp was cloned by RT-PCR and molecular cloning (Accession No.IN 164663). Its open reading frame was 882bp and encoded 293 amino acid residues. As performed in the nBLAST analysis, this gene had high homology with XTHs in other plant species, especially with compositae. The cluster analysis showed that the genetic distance between Tagetes Patula and dahlia, Chrysanthemum× morifolium, carnation was close. Thus TpXTH is conjectured to play an important role during the development of Tagetes Patula petals. The results lay the foundation for studying the molecular mechanism of TpXTH gene during development of Tagetes Patula petals.
机译：木糖葡聚糖内甘油糖苷酶/水解酶（Xth）是一种细胞壁松弛因子，已经证实与细胞延伸，水果软化，花瓣衰老等有关。在本研究中，为了讨论Compositae中的Xth基因的结构特征，棘爪的花瓣属于乙烯不敏感植物，用作实验材料。结果，通过RT-PCR和分子克隆克隆了具有906bp的Xth基因的cDNA（加入编号164663）。其开放阅读框架为882bp，编码293个氨基酸残基。如在NBlast分析中进行的，该基因在其他植物物种中具有高同源性，特别是与组合物。聚类分析表明，Tagetes Patula和Dahlia，Chrysanthemum×Morifolium，康乃馨的遗传距离是近的。因此，TPXth被猜测在Tagetes Patula花瓣的发展过程中发挥着重要作用。结果为研究TPXth基因在棘丁坪瓣的开发过程中奠定了基础。

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