• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Marine Biotechnology Conference >Molecular Characterization and Expression of α-Globin and β-Globin Genes in the Euryhaline Flounder (Platichthys flesus)
【24h】

Molecular Characterization and Expression of α-Globin and β-Globin Genes in the Euryhaline Flounder (Platichthys flesus)

机译：大戟比目鱼（Platichthys flesus）中α-球蛋白和β-球蛋白基因的分子表征和表达

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

@@ In order to understand the possible role of globin genes in fish salinity adaptation, we report the molecular characterization and expression of all four subunits of haemoglobin, and their response to salinity challenge in flounder. The entire open reading frames of α1-globin and α2-globin genes were 432 and 435 bp long, respectively, whereas the β1-globin and β2-globin genes were both 447 bp. Although the head kidney (pronephros) is the predicted major site of haematopoiesis, real-time PCR revealed that expression of α-globin and β-globin in kidney (mesonephros) was 1.5 times higher than in head kidney. Notably, the α1-globin and β1-globin mRNA expression was higher than α2-globin and β2-globin in kidney. Expression levels of all four globin subunits were higher in freshwater- (FW-) than in seawater- (SW-) adapted fish kidney. If globins do play a role in salinity adaptation, this is likely to be more important in combating the hemodilution faced by fish in FW than the dehydration and salt loading which occur in SW.
机译：为了了解球蛋白基因在鱼类盐度适应中的可能作用，我们报道了血红蛋白所有四个亚基的分子特征和表达，以及它们对比目鱼盐度挑战的反应。 α1-球蛋白和α2-球蛋白基因的整个开放阅读框分别长432和435bp，而β1-球蛋白和β2-球蛋白基因均为447bp。尽管头肾（前肾）是预测的造血功能的主要部位，但实时PCR显示，肾脏（中肾）中α-珠蛋白和β-珠蛋白的表达是头肾的1.5倍。值得注意的是，肾脏中α1-球蛋白和β1-球蛋白的mRNA表达高于α2-球蛋白和β2-球蛋白。在适应海水的鱼肾中，所有四个球蛋白亚基的表达水平在淡水（FW-）中均高于在海水（（SW-））中。如果珠蛋白确实在盐度适应中发挥作用，那么在对抗鱼类在鱼类体内所面临的血液稀释中，这可能比在西南部发生的脱水和盐分负荷更为重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号