Enzymatic Branched Assembly of P450cam with Putidaredoxin and Putidaredoxin Reductase Produces a Catalytically Competent Nanocatalyst

机译：P450CAM的酶分枝组装与普发玻璃和普发葡聚糖素还原酶产生催化态纳米催化剂

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@@Cytochrome P450s catalyze diverse types of reactions containing hydroxylations, epoxidations, dealkylations and heteroatom oxidations, which make P450s promising industrial catalysts. P450s require electrons from NAD(P)H to convert molecular oxygen into reactive species. However, excess amount of electron transfer partner proteins (more than several tens molar ratio of P450) is necessary to obtain sufficient reaction rate and coupling efficiency, and this is a bottleneck for practical application of P450s.

机译：@@细胞色素P450s催化含有羟基，环氧化，诸多氧化的各种类型的反应，这使得P450S有前途的工业催化剂。 P450S要求NAD（P）H的电子将分子氧转化为反应性物种。然而，对于获得足够的反应速率和偶联效率，需要过量的电子转移伴蛋白（P450的多于几十摩尔比），并且这是P450S实际应用的瓶颈。

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《China-Japan-Korea joint symposium on enzyme engineering》|2010年||共2页
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Kentaro Ichiman; Hidehiko Hirakawa;
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1. NMR studies of putidaredoxin: associations of putidaredoxin with NADH-putidaredoxin reductase and cytochrome P450cam [J] . Masaaki Aoki, Koichiro Ishimori, Isao Morishima Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology . 1998,第0期

机译：核苷还原酶的核磁共振研究：核苷还原酶与NADH-核苷还原酶还原酶和细胞色素P450cam的关联
2. Laser Flash Induced Electron Transfer in P450cam Monooxygenase: Putidaredoxin Reductase-Putidaredoxin Interaction [J] . Irina F. Sevrioukova, James T. Hazzard, Gordon Tollin, Biochemistry . 2001,第35期

机译：激光闪光诱导的P450cam单加氧酶中的电子转移：铂氧还蛋白还原酶-铂氧还蛋白相互作用
3. Isothermal titration calorimetric studies on the associations of putidaredoxin to NADH-putidaredoxin reductase and P450cam [J] . Masaaki Aoki, Koichiro Ishimori, Harumi Fukada, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology . 1998,第0期

机译：等温滴定量热法研究恶臭毒素与NADH-恶臭毒素还原酶和P450cam的关系
4. Enzymatic Branched Assembly of P450cam with Putidaredoxin and Putidaredoxin Reductase Produces a Catalytically Competent Nanocatalyst [C] . Kentaro Ichiman, Hidehiko Hirakawa China-Japan-Korea joint symposium on enzyme engineering . 2010

机译：P450cam的酶支化组装与Putidaredoxin和Putidaredoxin还原酶产生催化活性的纳米催化剂
5. Toward the design of an enzymatic breath sensor for acetone and the directed assembly of enzymes on comb-branched DNA. [D] . Hausmann, Nina Zulic. 2013

机译：旨在设计一种用于丙酮的酶促呼吸传感器，以及将酶直接定向排列在梳状分支DNA上。
6. Tricistronic Overexpression of Cytochrome P450cam Putidaredoxin and Putidaredoxin Reductase Provides a Useful Cell-based Catalytic System [O] . Donghak Kim, Paul R. Ortiz de Montellano -1

机译：细胞色素p450cam普发葡萄酒和普发籽蛋白还原酶的特异性过表达提供了一种有用的基于细胞的催化系统
7. Solution NMR Structure of Putidaredoxin–Cytochrome P450cam Complex via a Combined Residual Dipolar Coupling–Spin Labeling Approach Suggests a Role for Trp106 of Putidaredoxin in Complex Formation [O] . Wei Zhang, Susan S. Pochapsky, Thomas C. Pochapsky, 2008

机译：通过组合残留的残留偶极偶联 - 旋转旋转方法的普罗脂毒素 - 细胞色素P450CAM复合物的溶液NMR结构表明，普发葡聚糖素在复杂的形成中的作用表明了TRP106

Enzymatic Branched Assembly of P450cam with Putidaredoxin and Putidaredoxin Reductase Produces a Catalytically Competent Nanocatalyst

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