In recent years, polymer surfaces have become increasingly popular for biomolecule attachment due to their relatively low cost and desirable bulk physico-chemical characteristics. However, the chemical inertness of some polymer surfaces poses an obstacle to their successful implementation in a broader range of biomaterials applications. We describe the use of argon plasma to generate reactive hydroxyl moieties at the surface of polystyrene microtiter plates. The plates are then selectively functionalized with silanes and crosslinkers suitable for the covalent immobilization of biomolecules. We demonstrate that this protocol supports direct, covalent immobilization ofbiomoleculesonmicrotiterplates. Importantly, the biomolecules retained functionality and demonstrated attachment efficiency comparable to commercial pre-activated microtiter plates.
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