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首页> 外文会议>International Forum on Advanced Material Science and Technology;IFAMST-7 >Construction of Biosensor Based on Glassy Carbon Electrode Modified by Composite Film of Multi-Walled Carbon Nanotubes and Poly(ethylenimine)
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Construction of Biosensor Based on Glassy Carbon Electrode Modified by Composite Film of Multi-Walled Carbon Nanotubes and Poly(ethylenimine)

机译：多壁碳纳米管与聚乙烯亚胺复合膜修饰的玻碳电极生物传感器的构建

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Multi-walled carbon nanotubes (MWCNTs) were dispersed in an aqueous solution containing water-soluble poly(ethylenimine), and used to prepare MWCNTs modified glassy carbon electrode (GC/MWCNTs). A scanning electron microscopy was employed for characterizing the surface morphology of the modified electrode. The electrochemical behaviors of coenzyme (reduced nicotinamide adenine dinucleotide, NADH) on GC/MWCNTs were studied by both cyclic voltammetry and amperometry, and it is found clearly that the high overpotential for the direct oxidation of NADH on bare GC electrode was largely decreased in the presence of MWCNTs. The GC/MWCNTs electrode exhibited excellent properties as a platform for the construction of electrochemical biosensors based on NAD-dependent dehydrogenases, and an alcohol biosensor was successfully developed by immobilizing alcohol dehydrogenase on GC/MWCNTs.
机译：将多壁碳纳米管（MWCNT）分散在含有水溶性聚（乙烯亚胺）的水溶液中，并用于制备MWCNTs修饰的玻璃碳电极（GC / MWCNTs）。使用扫描电子显微镜表征改性电极的表面形态。通过循环伏安法和安培法研究了辅酶（还原的烟酰胺腺嘌呤二核苷酸，NADH）在GC / MWCNTs上的电化学行为，并且清楚地发现，裸露的GC电极上NADH直接氧化的高过电位大大降低了。 MWCNT的存在。 GC / MWCNTs电极具有优异的性能，可作为构建基于NAD依赖的脱氢酶的电化学生物传感器的平台，并且通过将醇脱氢酶固定在GC / MWCNTs上成功开发出了一种醇生物传感器。

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