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首页> 外文会议>Annual biochemical engineering symposium >Metabolic engineering of Catharanthus roseus hairy roots for terpenoid indole alkaloid production under light and dark conditions
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Metabolic engineering of Catharanthus roseus hairy roots for terpenoid indole alkaloid production under light and dark conditions

机译：在轻微和黑暗条件下，Catharanthus Roseus Hairy Roots的代谢工程为Terpenoid Indole生物碱生产

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The valuable antineoplastic alkaloids vincristine and vinblastine extracted from Catharanthus roseus have seen significant research effort towards increasing their production. Extensive work has shed light on the complex biosynthetic pathways and the strict multilevel regulation that controls metabolite levels and flux. Several enzymes in the later part of the pathway are also light-dependent and may be up- or down-regulated with light exposure. All of this complexity results in a challenging model for plant metabolic engineering. This work characterizes metabolic engineering efforts of the terpenoid indole alkaloid biosynthesis pathway within hairy root cultures of C. roseus at three locations: the indole alkaloid pathway entrance, the non-mevalon-ate/terpenoid alkaloid pathway entrance, and in the terpenoid indole alkaloid (TIA) pathway. The gene overexpression is controlled under a glucocorticoid-inducible promoter system and characterization is performed with metabolite measurements made 72 h after gene induction. The trans-genic lines were also light-adapted to investigate light-mediated regulation of the biosynthesis pathways. Results from this study reveal the levels of regulation in TIA synthesis and will guide further efforts in metabolic engineering.
机译：从Catharanthus Roseus中提取的有价值的抗肿瘤生物碱血管素和长春植物已经看到了增加其生产的显着研究努力。广泛的作品在复杂的生物合成途径和控制代谢物水平和助焊剂的严格的多级调节中脱光。在途径的后部部分中的几种酶也是光依赖性的，并且可以用曝光曝光来升级或下调。所有这些复杂性导致植物代谢工程有挑战性模型。这项工作表征了三个地区毛发根培养物中萜类吲哚生物碱生物合成途径的代谢工程努力：吲哚生物碱通路入口，非麦甲酸/萜类生物碱途径入口，以及萜类吲哚生物碱（ TIA）途径。基因过表达在糖皮质激素诱导型启动子体系下控制，并在基因诱导后72小时进行代谢物测量进行表征。转基因线也被轻适化，以研究生物合成途径的光介导的调节。本研究的结果揭示了TIA综合的调控水平，并指导了代谢工程的进一步努力。

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