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首页> 外文会议>World biomaterials congress >Synthesis and characterization of non-absorbable surgical sutures obtained from polyurethanes synthesized from polyols derived from castor oil, isophorone diisocyanate and poly(∈-caprolactone): physico-mechanical properties, degradability and in vitro cytotoxicity
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Synthesis and characterization of non-absorbable surgical sutures obtained from polyurethanes synthesized from polyols derived from castor oil, isophorone diisocyanate and poly(∈-caprolactone): physico-mechanical properties, degradability and in vitro cytotoxicity

机译：由蓖麻油，异佛酮二异氰酸酯和聚（甲己内酯）的多元醇合成的聚氨酯中获得的非可吸收手术缝合线的合成及表征：物理机械性能，可降解性和体外细胞毒性

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Studies on polyurethanes increased their interest in the biomedical field due to its properties characteristics, among which we can mentioned elastic properties, degradability and biocompatibility. In this project polyols were synthesized via modification of castor oil to obtain more functionality and more suitable monomers for the synthesis of polyurethanes, which can vary the hydrophilic/hydrophobic chemical nature of the material and thereby control its biocompatibility. Sutures were obtained by a spinning process using spinneret technique adapted for polyurethanes synthesized from polyols (derived from castor oil), isophorone diisocyanate and poly(e-caprolactone) at 5,10 and 20% w/w respectively. The physicochemical characterization of the sutures was performed using Fourier Transform Infrared spectroscopy (FTIR), mechanical properties such as stress-strain (D2256 / D2256M-10) and thermogravimetric analysis (TGA) along with morphologic characterization by scanning electron microscopy (SEM). These properties were correlated with in vitro degradability evaluated by simulated body fluid (SBF), phosphate buffered saline (PBS), and in vitro biocompatibility. The biocompatibility was analyzed by: ⅰ) in vitro cytotoxicity assays according to the ISO 10993-5 method, which is based on the metabolic reduction of bromide 3- (4,5-dimethylthiazol-2-yl) -2,5-difeniltetrazol (MTT) using mouse embryonic fibroblast cell line NIH/3T3 (ATCC CRL-1658), and ⅱ) the antimicrobial activity against E. coli based on the type of polyol and additive used in the suture synthesis. The characteristics of the synthetized sutures were very similar to the commercial surgical sutures. Chemical modification of castor oil and incorporation of poly(ε-caprolactone) did not generate a cytotoxic effect on the evaluated cell lines. The increase of the poly(e-caprolactone) concentration showed higher cell adhesion against the sutures, which exhibited bacterial inhibition against E. coli depending to its percentage of poly(e-caprolactone) and polyols. The evaluated materials are suggested as candidates to obtain non-absorbable surgical sutures due to their mechanical properties and biocompatibility presented in untransformed cells.
机译：由于其性质特征，对聚氨酯的研究增加了它们对生物医学领域的兴趣，其中我们可以提及弹性性质，可降解性和生物相容性。在该项目中，通过改变蓖麻油来获得多元醇，以获得更多的功能和更合适的单体用于合成聚氨酯，这可以改变材料的亲水/疏水化学性质，从而控制其生物相容性。通过使用Spinneret技术的纺丝方法获得缝合，所述喷丝工艺适用于从多元醇（来自蓖麻油），异佛酮二异氰酸酯和聚（E-己内酯）分别在5,10和20％w / w中合成的聚氨酯。使用傅里叶变换红外光谱（FTIR），诸如应力 - 菌株（D2256 / D2256M-10）和热重分析（TGA）的机械性能以及通过扫描电子显微镜（SEM）的形态学表征缝合物的物理化学表征。这些性质与通过模拟体液（SBF），磷酸盐缓冲盐水（PBS）和体外生物相容性评价的体外可降解性相关性。通过ISO 10993-5方法分析了生物相容性：Ⅰ）体外细胞毒性测定法，其基于溴化物3-（4,5-二甲基噻唑-2-基）-2,5- difeNIntroTrazol的代谢还原（ MTT）使用小鼠胚胎成纤维细胞系NIH / 3T3（ATCC CRL-1658），Ⅱ）基于缝合物合成中使用的多元醇和添加剂的类型对大肠杆菌进行抗微生物活性。合成缝合线的特性与商业手术缝合线非常相似。蓖麻油的化学改性和聚（ε-己内酯）的掺入没有对评估的细胞系产生细胞毒性作用。聚（E-己内酯）浓度的增加表现出对缝合线的较高细胞粘附，这取决于其聚（己内酯）和多元醇的百分比表现出对大肠杆菌的细菌抑制。提出评估的材料作为在未转化细胞中呈现的机械性能和生物相容性，以获得不可吸收的手术缝合线的候选物。

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