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Copper oxide nanoparticles affect mechanical properties and viability of cultured human airway smooth muscle cells

机译：氧化铜纳米粒子影响培养的人气道平滑肌细胞的机械性能和生存能力

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The booming nanotechnology produces a large number of airborne particles. Some of these nanoparticles come into contact with respiratory tract and even penetrate through the epithelium barrier. Such airborne particle exposure brings adverse effects to the respiratory system and further affects human health. Therefore, there has been increasing research focused on the interaction between particles and cells within the airway. Here we studied particle induced changes in human airway smooth muscle cells in relation to particle size, dose and exposure time. Materials and Methods: We incubated Type Ⅱ epithelial cells with various concentrations (2.5ug/ml, 5ug/ml,25ug/ml and 50ug/ml) of different sized (fine-5um, ultrafine-50nm) copper oxide (CuO) by submersed exposure for 24 h or 48 h. Then we collected the medium to culture human airway smooth muscle cells(HASMCs) for 24 h so that those CuO particles did not interact with HASMCs directly. We used Alamar Blue assay to measure cell viability and optical magnetic twisting cytometry to measure cell stiffness and agonist-induced cell contractility. Results: CuO particle exposure induced a decrease in viability, stiffness and contractility of human airway smooth muscle cell. The extent of the decline was closely associated with exposure time and particle concentration. At the same concentration, ultrafine particles showed a stronger influence on the measured parameters. This study demonstrates an indirect effect of nano-and micro- CuO particles on HASMCs. Due to smaller size and larger surface area per unit mass, CuO nanoparticles cause greater damages on cell viability, stiffness and contractility than CuO microparticles. In conclusion, CuO particles, particularly nano-sized are more toxic to airway and more likely to lead to dysfunction of airway biophysical properties.
机译：蓬勃发展的纳米技术会产生大量的空气传播颗粒。这些纳米粒子中的一些会与呼吸道接触，甚至穿透上皮屏障。此类空气传播的颗粒物暴露会对呼吸系统产生不利影响，并进一步影响人体健康。因此，越来越多的研究集中在气道内颗粒与细胞之间的相互作用上。在这里，我们研究了颗粒诱导的人气道平滑肌细胞与颗粒大小，剂量和暴露时间有关的变化。材料和方法：我们通过潜入不同浓度（2.5μg/ ml，5μg/ ml，25μg/ ml和50μg/ ml）的不同尺寸（细5um，超细50nm）氧化铜（CuO）的Ⅱ型上皮细胞进行培养。暴露24小时或48小时。然后，我们收集培养基以培养人气道平滑肌细胞（HASMC）24小时，以使这些CuO颗粒不会直接与HASMCs相互作用。我们使用Alamar Blue分析法来测量细胞活力，并使用光磁扭转细胞仪来测量细胞刚度和激动剂诱导的细胞收缩性。结果：CuO颗粒暴露导致人气道平滑肌细胞活力，硬度和收缩力下降。下降的程度与暴露时间和颗粒浓度密切相关。在相同的浓度下，超细颗粒对测量的参数显示出更强的影响。这项研究证明了纳米和微米级的CuO颗粒对HASMC的间接作用。由于更小的尺寸和每单位质量的更大的表面积，与CuO微粒相比，CuO纳米颗粒对细胞生存力，刚度和收缩力的损害更大。总之，CuO颗粒，特别是纳米颗粒，对气道的毒性更大，更可能导致气道生物物理特性的功能障碍。

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