In this paper, we present the modeling, design, and dynamic characterization of a flexure-based vibrating blade microtome that achieves submicron precision with minimized parasitic motions in multiple axes. The completed microtome is then integrated with a two-photon microscope (TPM) to form a serial two-photon tomography system for whole organ imaging. The automated system can obtain 3-D organ image at the speed of 10 GB/hr with subcellular resolution.
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