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首页> 外文会议>Society for Biomaterials annual meeting and exposition >Atomic Force Microscopy Study of Coagulation Factor XII Interaction with Factor XI Autoactivation on Hydrophilic and Hydrophobic Surfaces
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Atomic Force Microscopy Study of Coagulation Factor XII Interaction with Factor XI Autoactivation on Hydrophilic and Hydrophobic Surfaces

机译：亲水性和疏水性表面上凝血因子XII与因子XI自活化的相互作用的原子力显微镜研究

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Blood coagulation resulting from contact activation due to blood - material interactions remains a challenge in the use of blood-contacting devices. The initiating step of the intrinsic pathway of plasma coagulation cascade is widely accepted to be surface contact activation of the blood zymogen FXⅡ (Hageman Factor) into an active-enzyme form. In plasma, increased contact efficiency is seen with with anionic, hydrophilic procoagulants. However, recent work demonstrates that hydrophobic and hydrophilic activators have nearly equal autoactivation properties in neat buffer solutions of FXⅡ. These mechanisms remain unclear and motivate the further exploration of molecular interactions of FXⅡ with other proteins such as coagulation factor FXⅠ. In this study, AFM was employed to measure the interactions of activated FXⅡ with FXⅠ, with activated FXⅡ produced by interactions with hydrophilic (mica) and hydrophobic (HOPG) surfaces.
机译：在使用血液接触装置时，由于血液与物质的相互作用而引起的接触活化引起的血液凝结仍然是一个挑战。血浆凝血级联反应的内在途径的起始步骤被广泛接受为将血液酶原FXⅡ（Hageman因子）转化为活性酶形式的表面接触活化。在血浆中，可以看到阴离子，亲水性促凝剂的接触效率提高。然而，最近的研究表明，疏水性和亲水性活化剂在FXⅡ的纯缓冲溶液中具有几乎相同的自活化特性。这些机制尚不清楚，并促使人们进一步探索FXⅡ与其他蛋白，例如凝血因子FXⅠ的分子相互作用。本研究采用原子力显微镜测量活化的FXⅡ与FXⅠ的相互作用，以及通过与亲水（云母）和疏水（HOPG）表面相互作用而产生的活化FXⅡ的相互作用。

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