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Chapter 8 Sample-Independent Expression Stability Analysis of Human Housekeeping Genes Using the GeNORM Algorithm

机译:第8章使用Genorm算法对人类家务基因的独立样本表达稳定性分析

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The quantification of mRNAs has been used with great success in many medical research techniques. All of them can use housekeeping genes as internal standards. While most of the commonly used housekeeping genes may have varied expression stability in different human tissue samples or experimental conditions. In this study, 566 housekeeping genes were investigated by conducting a statistical analysis on a large human genome microarray database. The sample-independent expression stability value of every gene was calculated and ranked by using the GeNORM algorithm. Furthermore, microarray expression data of another mammalian model were used to evaluate the variation coefficient of the candidate genes expressed in the mouse models. Most of the candidate housekeeping genes exhibited similar expression stabilities in the two models. This analysis presents the sample-independent expression stability of a set of housekeeping genes.
机译:MRNA的量化已经在许多医学研究技术中取得了巨大的成功。所有这些都可以使用家务基因作为内部标准。虽然大多数常用的家务基因在不同的人体组织样本或实验条件下可能具有不同的表达稳定性。在本研究中,通过对大型人类基因组微阵列数据库进行统计分析来研究566个家庭基因。通过使用基因仪算法计算并排序每个基因的依赖性表达稳定性值。此外,使用另一种哺乳动物模型的微阵列表达数据来评估小鼠模型中表达的候选基因的变异系数。大多数候选家务基因基因在两种模型中表现出类似的表达稳定性。该分析介绍了一组内政基因的独立式表达稳定性。

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