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首页> 外文会议>International symposium on IT in medicine and education >Construction of Expression Vector of miRNA Specific for FUT3 and Identification of Its Efficiency in KATO-Ⅲ Gastric Cancer Cell Line
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Construction of Expression Vector of miRNA Specific for FUT3 and Identification of Its Efficiency in KATO-Ⅲ Gastric Cancer Cell Line

机译：FUT3特异性miRNA表达载体的构建及其在胃癌细胞系Kato-Ⅲ胃癌中效率的鉴定

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摘要

Human blood group Lewis antigen, which is fucosylated glycoconju-gates involved in the development of several pathologies. Fucosyltransferases are enzymes that add fucose to precursor glycan structures: FUT3 catalyze the addition of fucose to the α 1-3, 4 position and are detected in epithelial cells. As the expression of Lewis a is mainly controlled by FUT3, which can influence Lewis a'S synthesis, we choose FUT3 as our target gene to silence. In our study, we constructed FUT3-specific miRNA expression vector successfully. It showed in the experiment that FUT3-specific miRNA expression vector could actively inhibit FUT3'S expression in mRNA level in KATO-Ⅲ gastric cancer cell line, and decrease Lewis antigen'S synthesis as well growth. From this experiment, we obtain the best mi RNA sequence of FUT3, which may serve as a new strategy for investigating the mechanism of molecular glycol-pathology of gastric cancer targeted tumor gene therapy.
机译：人血液组Lewis抗原，其是岩藻糖苷化的甘油膜栅，参与了几种病理的发育。岩藻糖基转移酶是将岩藻糖添加到前体聚糖结构的酶：Fut3催化在α1-3，4个位置的添加岩藻糖，并在上皮细胞中检测。由于Lewis A的表达主要由Fut3控制，这可以影响Lewis A的合成，我们选择Fut3作为我们的目标基因沉默。在我们的研究中，我们成功地构建了Fut3特异性miRNA表达载体。它在实验中显示了Fut3特异性miRNA表达载体可以积极抑制Cut3在Kato-Ⅲ胃癌细胞中MRNA水平中的表达，并降低Lewis抗原的合成效果。从这个实验来看，我们获得Fut3的最佳Mi RNA序列，其可以作为研究胃癌靶向肿瘤基因治疗的分子二醇病理机制的新策略。

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