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Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox)

机译:Wintersweet(Chimonanthus Praecox)硫酯酶的分子克隆,序列分析

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A coding region of wintersweet cDNA was cloned via screening a wintersweet cDNA library. The CpFATB cDNA is 1110 bp in length with an open reading frame (ORF) of 1107 bp encoding a protein of 369 amino acids. Molecular weight and isoelectric point of the protein is 41.72 kD and 7.72, respectively. Bioinformatics analysis shows that a signal peptide with 49 amino acid residues and Protein Localization Sites exists in the chloroplast stroma. This classifies the protein as stable.
机译:通过筛选冬季甜cDNA文库,克隆了冬季甜cDNA的编码区。 CpFATB cDNA的长度为1110 bp,开放阅读框(ORF)为1107 bp,编码369个氨基酸。该蛋白质的分子量和等电点分别为41.72 kD和7.72。生物信息学分析表明,叶绿体基质中存在一个具有49个氨基酸残基和蛋白质定位位点的信号肽。这将蛋白质分类为稳定的。

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