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首页> 外文会议>Annual conference of the International Society of Exposure Science >Synergistic and Hermetic Effects of Volatile Organic Compounds on Marine Luminescent Bacterium Vibrio fischeri
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Synergistic and Hermetic Effects of Volatile Organic Compounds on Marine Luminescent Bacterium Vibrio fischeri

机译：挥发性有机化合物对海洋发光细菌费氏弧菌的协同和密封作用

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Various chemical contaminants present including unknown and unidentified pollutants in indoor environment. These toxicities as mixture have not been evaluated with any regard for the possibility of serious impacts on the environment. This study aimed to find the influence on bioluminescence of luminescent bacterium Vibrio fishceri, when typical indoor air pollutants (VOCs) were mixed, compared with the case of single components. Bioassay using V. fischeri is well known as a novel screening method for toxicity evaluation of the environmental contamination. Both single and mixed VOC solutions by two kinds of VOCs were prepared in various concentration levels with dilution by methanol as a solvent. Acetaldehyde, formaldehyde, toluene and ethyl benzene were tested as typical VOCs. Each sample solution (50 μL) was mixed into 2 ml of reactivated bacterial solution in a well of 24-well plate. Then, time course of relative bioluminescence intensity (BLI) was measured by a luminometer with blank and positive control solution. Then, EC50 of single components were obtained from dose-response curves and mixed toxic properties were evaluated with mixture toxicity index, MTI. As a result, every mixture showed significant increasing or decreasing of toxicity compared with the cases of single VOCs probably because of synergistic effect. On the other hand, biostimulation was observed in the low concentration ranges of each VOC, even these components remarkably inhibited the bioluminescence at high concentrations. This result probably indicates the hermetic effect for VOCs. This study suggests that some combinations of VOCs have more serious or trivial impacts on marine luminescent bacterium than previously recognized.
机译：存在各种化学污染物，包括室内环境中的未知和未知污染物。尚未评估这些毒性的混合物是否会对环境造成严重影响。这项研究的目的是发现与单一成分相比，典型的室内空气污染物（VOC）混合时对发光细菌鱼弧菌的生物发光的影响。众所周知，使用费氏弧菌的生物测定是一种新的筛选方法，用于评估环境污染的毒性。在甲醇作为溶剂稀释的情况下，制备了两种浓度不同的单一和两种挥发性有机化合物的挥发性有机化合物溶液。测试了乙醛，甲醛，甲苯和乙苯作为典型的VOC。在24孔板的孔中，将每种样品溶液（50μL）混合到2 ml的重新活化的细菌溶液中。然后，通过具有空白和阳性对照溶液的发光计测量相对生物发光强度（BLI）的时程。然后，从剂量-反应曲线获得单一成分的EC50，并用混合物毒性指数MTI评估其混合毒性。结果，与单一VOC相比，每种混合物均显示出明显的毒性增加或降低，这可能是由于协同作用所致。另一方面，在每种VOC的低浓度范围内观察到生物刺激，即使这些组分在高浓度下也显着抑制了生物发光。该结果可能表明了VOC的气密作用。这项研究表明，VOC的某些组合对海洋发光细菌的影响比以前公认的更为严重或微不足道。

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