Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

机译：通过移植的人骨髓基质细胞筛选成骨组合盒

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We have developed a combinatorial platform for high-throughput in vivo screening of osteogenesis by hBMSCs on various scaffold formulations. Typically, a mouse can receive 4 subcutaneous implantations. The combi-cassette increases this to 7 implants, increasing efficiency and reducing animal to animal variability. In addition, each well in the combi-cassette uses 66% less material (cells and scaffold) than the traditional "non-combi" transplants. The combi-cassette also makes for efficient sample handling since the whole cassette can be fixed, embedded and stained at once and all 7 transplants appear on one slide. This approach also has the ethical benefit of reducing the number of animals required for each test.

机译：我们已经开发了一种组合平台，可通过hBMSC对各种支架制剂进行高通量体内筛查成骨作用。通常，小鼠可以接受4次皮下植入。组合式磁带将其增加到7个植入物，从而提高了效率并减少了动物之间的变异性。此外，与传统的“非复合”移植相比，组合式磁带中的每个孔使用的材料（细胞和支架）减少了66％。组合式磁带盒还可以实现高效的样品处理，因为整个盒式磁带可以立即固定，包埋和染色，并且所有7种移植物都出现在一张载玻片上。这种方法还具有减少每次测试所需动物数量的伦理益处。

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《Society for Biomaterials annual meeting and exposition》|2014年|305-305|共1页
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Subhadip Bodhak; Luis Fernandez de Castro Diaz; Sergei A. Kuznetsov; Tina Kilts; Marian F. Young; Sheng Lin-Gibson; Pamela G. Robey; Carl G. Simon Jr.;
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1. Retrovirally transduced bone marrow stromal cells isolated from a mouse model of human osteogenesis imperfecta (oim) persist in bone and retain the ability to form cartilage and bone after extended passaging. [J] . Oyama M, Tatlock A, Fukuta S, Gene therapy . 1999,第3期

机译：从人类成骨不全症（oim）小鼠模型中分离出的逆转录病毒转导的骨髓基质细胞在骨骼中持续存在，并在长时间传代后保留形成软骨和骨骼的能力。
2. Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches [J] . Hempel Ute, Mueller Katrin, Preissler Carolin, Stem cells international . 2016,第7期

机译：人骨髓间质细胞：用于体外骨肉骨质发生和骨组织工程方法的可靠，具有挑战性的工具
3. Ectopic osteogenesis with immortalized human bone marrow stromal stem cells and heterologous bone [J] . Yong Teng, Yunyu Hu, Xusheng Li, Journal of Applied Biomedicine . 2011,第2期

机译：具有永生化的人骨髓基质干细胞和异源骨的异位成骨
4. Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells [C] . Subhadip Bodhak, Luis Fernandez de Castro Diaz, Sergei A. Kuznetsov, Society for Biomaterials annual meeting and exposition . 2014

机译：用于筛选移植的人骨髓基质细胞筛选成骨的组合盒
5. Rapidly self-renewing stem cells in human bone marrow: The identification, characterization and regulation of a unique bone marrow stromal cell sub-population. [D] . Colter, David Christopher. 2001

机译：人类骨髓中快速自我更新的干细胞：独特的骨髓基质细胞亚群的鉴定，表征和调节。
6. Human Bone Marrow Stromal Cells: A Reliable Challenging Tool for In Vitro Osteogenesis and Bone Tissue Engineering Approaches [O] . Ute Hempel, Katrin Müller, Carolin Preissler, 2016

机译：人骨髓基质细胞：一种可靠的具有挑战性的体外成骨和骨组织工程方法的工具
7. Retrovirally transduced bone marrow stromal cells isolated from a mouse model of human osteogenesis imperfecta (oim) persist in bone and retain the ability to form cartilage and bone after extended passaging [O] . M Oyama, A Tatlock, S Fukuta, 1999

机译：逆转录病毒转导的骨髓基质细胞中分离的人骨质骨质发生缺乏细胞（OIM）持续存在于骨骼中并保留在延长传递后形成软骨和骨的能力

Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

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