Establishment of a Method to Measure the Interaction Between Nedd4 and UbCH5c for Drug Screening

机译：建立Nedd4与UbCH5c相互作用的药物筛选方法的建立

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Protein ubiquitination is closely associated with tumorigenesis. E3 ubiquitin ligase Nedd4 is overexpressed in prostate, bladder, and colorectal cancer. Nedd4 interacts with UbCH5c ubiquitin transferase through its HECT domain. Two GST-tagged Nedd4 proteins and His-HA-UbCH5c protein are expressed and purified. Using these recombinant proteins, a method is established to measure the interaction between Nedd4 and UbCH5c. This established method can be used to screen the small molecule inhibitors to block the interaction between Nedd4 and UbCH5c, which inhibit the activity of E3 ubiquitin ligase Nedd4.

机译：蛋白质泛素化与肿瘤发生密切相关。 E3泛素连接酶Nedd4在前列腺癌，膀胱癌和结肠直肠癌中过表达。 Nedd4通过其HECT结构域与UbCH5c泛素转移酶相互作用。表达并纯化了两个带有GST标签的Nedd4蛋白和His-HA-UbCH5c蛋白。使用这些重组蛋白，建立了一种测量Nedd4与UbCH5c之间相互作用的方法。此建立的方法可用于筛选小分子抑制剂，以阻断Nedd4与UbCH5c之间的相互作用，从而抑制E3泛素连接酶Nedd4的活性。

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《International conference on applied biotechnology》|2015年|297-303|共7页
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Kunyuan Kou; Jianli Dang; Baoxia Zhang; Guanrong Wu; Yuyin Li; Aipo Diao;
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Nedd4; UbCH5c; Protein purification; Protein interaction;

机译：Nedd4; UbCH5c;蛋白质纯化;蛋白质相互作用;

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Establishment of a Method to Measure the Interaction Between Nedd4 and UbCH5c for Drug Screening

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