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To Establish the Regeneration System of Sweet Sorghum Immature Embryos

机译:建立甜高粱未成熟胚的再生体系

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Sweet sorghum is an important forage and energy crop. In this experiment, sweet sorghum immature embryos were taken to be explants, through inducting and regenerating for sweet sorghum callus of different genotypes, to obtain the best culture medium formula and create appropriate conditions for establishing efficient regeneration system. The result showed that, for callus induction, the response of immature embryos varied with the genotype, but the medium is not. Of these, Xinliang 52 showed the highest frequency of callus induction under appropriate culture conditions. As regards regenerable callus formation, the response of callus varied with the genotype and medium. 07-27 regeneration occurred at high frequencies when MSR and MBR were added in the regeneration medium. And there is nothing on NBI medium for the whole genotypes. So NBI is not suitable for callus regeneration culture.
机译:甜高粱是重要的牧草和能源作物。本实验以甜高粱未成熟胚为外植体,通过诱导和再生不同基因型的甜高粱愈伤组织,获得最佳培养基配方,为建立有效的再生体系创造了适宜的条件。结果表明,对于愈伤组织的诱导,未成熟胚的反应随基因型而变化,而培养基却没有。其中,新良52在适当的培养条件下显示出最高的愈伤组织诱导频率。关于可再生愈伤组织的形成,愈伤组织的反应随基因型和培养基而变化。当在再生介质中添加MSR和MBR时,以高频率发生07-27再生。整个基因型在NBI培养基上都没有。因此,NBI不适合用于愈伤组织再生培养。

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