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首页> 外文会议>International Conference on Bioinformatics and Biomedical Engineering >The Enhancement of drug sensitivity of human AFP-producing gastric carcinoma cell line FU97 to arsenic trioxide Using AFP as targeting siRNA
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The Enhancement of drug sensitivity of human AFP-producing gastric carcinoma cell line FU97 to arsenic trioxide Using AFP as targeting siRNA

机译：使用AFP作为靶向siRNA使用AFP产生人AFP胃癌细胞系FU97对砷三氧化砷的药物敏感性

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AFP-producing gastric carcinoma (APGC) are known to have a poorer prognosis and to show a higher incidence of liver metastasis than ordinary gastric carcinoma. Therefore, surgical resection, chemotherapy or radiotherapy is usually not satisfactory. In recent years, effects of arsenic trioxide (As_2O_3) on cell proliferation and induced apoptosis of solid tumors have been widely studied, while little is known about the APGC. This study was undertaken to delineate the effect of As_2O_3 on AFP gene expression, the cell growth and apopotosis, and to study the effect of the siRNA targeting AFP gene on the drug sensitivity of APGC cell line FU97 to As_2O_3. AFP gene sequence-specific siRNA expression vector (AFP-siRNA) and sequence-unrelated control were transfected into FU97. At 6h after transfection, the cells were cultured with 2 μmol/L As_2O_3, with corresponding untreated cells serving as controls. The growth inhibition rate on FU97 was determined by MTT assay; Apoptosis of FU97 was investigated by DNA gel electrophore; The expressions of AFP, VEGF were determined by realtime-PCR, western blot, ELISA and chemistry luminescence. The combination of AFP-siRNA with arsenic trioxide resulted in significantly inhibitory action on growth of FU97 cells, as well as descending of expression of AFP and VEGF. The effective siRNA targeting AFP can increase the drug sensitivity of the FU97 to arsenic trioxide.
机译：已知AFP胃癌（APGC）具有较差的预后，并且显示出比普通胃癌的肝转移发病率更高。因此，手术切除，化疗或放射疗法通常不令人满意。近年来，广泛研究了三氧化砷（AS_2O_3）对细胞增殖和诱导的固体肿瘤凋亡的影响，但对于APGC而言，较少的人。这项研究进行划定三氧化二砷对AFP基因表达，细胞生长和中细胞凋亡的效果，并研究siRNA的APGC细胞系FU97的药物敏感性三氧化二砷上靶向AFP基因的效果。将AFP基因序列序列的siRNA表达载体（AFP-siRNA）和序列 - 不相关对照转染到FU97中。在转染后6h，用2μmol/ las_2O_3培养细胞，用相应的未处理的细胞用作对照。通过MTT测定法测定FU97上的生长抑制率; DNA凝胶电泳研究了FU97的凋亡; AFP，VEGF的表达由RealTime-PCR，Western印迹，ELISA和化学发光确定。 AFP-的siRNA的三氧化二砷的组合导致对FU97细胞的生长，以及AFP和VEGF的表达的下降显著抑制作用。靶向AFP的有效siRNA可以将FU97的药物敏感性提高到砷三氧化砷。

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