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首页> 外文会议>International Conference on Bioinformatics and Biomedical Engineering >Detection of Haplosporidium nelsoni in oysters from China coast
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Detection of Haplosporidium nelsoni in oysters from China coast

机译：从中国海岸牡蛎中纳尔尼纳尔尼的检测

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Oysters of nineteen cities from China coast were investigated in our study. The localization of Haplosporidium nelsoni-like (MSX) plasmodia was detected by light microscopy. In Situ hybridization of infected tissue section was conducted with the H.nelsoni-specific DNA probe MSX1347. DNA was extracted from the oysters Crassostrea gigas, Crassostrea plicatula, Crassostrea rivularis, Ostrea crenulifera for PCR amplification. MSX-A', MSX-B and Hap-F_2, Hap-R_2 were applied to amplify the MSX. PCR amplification of genomic DNA with primer pairs MSX-A'+B and HAP-F_2+R_2 yielded the expected products for 573bp, 239bp, respectively. These PCR products were sequenced and the similarities from C. gigas was 99% in the 16S-like rRNA region of H.nelsoni. Subsequently, MSX was identified in Crassostrea Plicatula. However, MSX was not detected in oysters, C. rivularis, O. crenulifera. Our study demonstrates H. nelsoni in oysters presents between 24 and 40 at north latitude in China. These results approved that H. nelsoni occured in China and the distribution of MSX could be relative to host and latitude.
机译：中国沿岸的十九个城市的牡蛎在我们的研究中进行了调查。通过光学显微镜检测Haplosperianium样（MSX）纤维素的定位。原位杂交的受感染的组织切片用H.CleSoni特异性DNA探针MSX1347进行。从牡蛎鲫鱼，Crassostraa Plicatula，Crassostrea rivularis，Ostrea Crenulifera提取DNA，用于PCR扩增。 MSX-A'，MSX-B和HAP-F_2，HAP-R_2被应用于放大MSX。具有引物对MSX-A'+ B和HAP-F_2 + R_2的基因组DNA的PCR扩增分别产生573bp，239bp的预期产物。这些PCR产物测序，在H.Nelsoni的16S样RRNA区域中，来自C.Gigas的相似性为99％。随后，MSX在Crasstrea Plicatula中鉴定。然而，在牡蛎，C.Rivularis，O. Crenulifera未检测到MSX。我们的研究表明，牡蛎中的H. Nelsoni在中国北纬24至40岁之间。这些结果批准在中国发生的H. Nelsoni和MSX的分布可以相对于主持人和纬度。

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