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Transmission SPR of Gold Nanoslit Array and Ultrasensitive Detection of A Retinol Binding Protein

机译:金纳米纳米阵列的透射SPR和视黄醇结合蛋白的超声检测

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Retinal-modified SAM-coated nanoslit array thin gold film was used for ultra-sensitive (picomolar) detection of a ligand protein (β-Lactoglobulin) with a transmission SPR mode. The transmission spectra of nanoslit arrays with varying periods were evaluated for nanostructure optimization with respect to primary peak position, shape and sensitivity. Sensitivity of this method, as measured in water, was estimated at 3 ng/mL, or approximately 130 picomolar. Additionally a monotonic shift in primary peak wavelength was observed with changing nanoslit periodicity. This type of in-plane nanoarray chip is currently being integrated with microfluidic platforms for chemical and/or biological sensing applications.
机译:视网膜改性的SAM涂覆的纳米溶液阵列薄金膜用于具有透射SPR模式的Uligand(Picomolar)检测配体蛋白(β-乳酰叶蛋白)。评价具有不同周期的纳米玻璃阵列的透射光谱,用于相对于初级峰位置,形状和灵敏度的纳米结构优化。如在水中测量的该方法的敏感性估计在3ng / ml,或约130双摩尔。另外,观察到初级峰值波长的单调转变,随着纳米半球周期性而观察到。这种类型的面内纳米阵列芯片目前与用于化学和/或生物传感应用的微流体平台集成。

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