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Identification of column edges of DNA fragments by using K-means clustering and mean algorithm on lane histograms of DNA agarose gel electrophoresis images

机译:利用K-均值聚类和DNA琼脂糖凝胶电泳图像泳道直方图的均值算法识别DNA片段的柱边缘

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Gel electrophoresis (GE) is one of the most used method to separate DNA, RNA, protein molecules according to size, weight and quantity parameters in many areas such as genetics, molecular biology, biochemistry, microbiology. The main way to separate each molecule is to find borders of each molecule fragment. This paper presents a software application that show columns edges of DNA fragments in 3 steps. In the first step the application obtains lane histograms of agarose gel electrophoresis images by doing projection based on x-axis. In the second step, it utilizes k-means clustering algorithm to classify point values of lane histogram such as left side values, right side values and undesired values. In the third step, column edges of DNA fragments is shown by using mean algorithm and mathematical processes to separate DNA fragments from the background in a fully automated way. In addition to this, the application presents locations of DNA fragments and how many DNA fragments exist on images captured by a scientific camera.
机译:凝胶电泳(GE)是在遗传学,分子生物学,生物化学,微生物学等许多领域中根据大小,重量和数量参数分离DNA,RNA,蛋白质分子的最常用方法之一。分离每个分子的主要方法是找到每个分子片段的边界。本文介绍了一个软件应用程序,该程序以3个步骤显示DNA片段的列边缘。在第一步中,该应用程序通过基于x轴进行投影来获得琼脂糖凝胶电泳图像的车道直方图。在第二步中,它利用k均值聚类算法对车道直方图的点值进行分类,例如左侧值,右侧值和不需要的值。在第三步中,通过使用均值算法和数学过程以全自动方式将DNA片段与背景分离,显示了DNA片段的柱边缘。除此之外,该应用程序还可以显示DNA片段的位置以及科学相机拍摄的图像上存在多少DNA片段。

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