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Effect of magnetic attraction on gene transfection of bacteria

机译：磁引力对细菌基因转染的影响

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We propose cells can be descended using magnetic attraction, which leads to shorter experimental time and higher efficiency. We used an electroporation chip with adjustable electromagnetic field as the experimental platform, and tested Escherichia coli and 6-nm magnetic beads combined with DNA plasmid. The magnetic beads were positively charged and easy to bind to the negatively charged cell membrane of E. coli. The magnetic beads and E. coli could be attracted quickly to the bottom because of the electromagnet and could reduce operational time and enhance transfection efficiency. After electroporating and culturing, we obtained the results for E. coli with drug resistance and calculated the number of colony as the transfection efficiency. The achieved transfection efficiency using magnetic beads was seven-fold higher than that without magnetic bead. The following optimum parameter values were determined: 1.4 × 10 bead/ml for nano-magnetic bead concentration, 200 Gauss for magnetic flux density, and 40 s for magnetic attraction lasting time. The results will help develop transfection applications for low-descent-velocity cells.
机译：我们建议细胞可以利用磁引力下降，这导致更短的实验时间和更高的效率。我们使用具有可调电磁场的电穿孔芯片作为实验平台，并测试了大肠杆菌和结合了DNA质粒的6 nm磁珠。磁珠带正电，易于与大肠杆菌的带负电的细胞膜结合。电磁珠可以将电磁珠和大肠杆菌迅速吸引到底部，并且可以减少操作时间并提高转染效率。经过电穿孔和培养后，我们获得了具有抗药性的大肠杆菌结果，并计算了菌落数作为转染效率。使用磁珠的转染效率比不使用磁珠的转染效率高七倍。确定了以下最佳参数值：纳米磁珠浓度为1.4×10珠/ ml，磁通量密度为200高斯，磁吸持续时间为40 s。该结果将有助于开发低速细胞的转染应用。

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