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High-density distributed electrodes network for generation of murine induced pluripotent stem cells

机译:高密度分布式电极网络可产生鼠类诱导性多能干细胞

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High-density electrodes network (HDEN) demonstrates a flexible electroporation procedure for gene transfection in different cell types. In this study, the HDEN device shows its capacity as a great candidate in optimization of efficiency in non-viral induction of murine induced pluripotent stem cells (iPSCs) by demonstrating successful expression of different kinds of transgenes of fluorescent proteins. High and steady expression of iPSCs gene expression has been observed. A reasonable transfection rate and viability in different cell types have been demonstrated as well. It implies that a new alternative of iPSCs generation with a combination of plasmid DNA induction and HDEN may be feasible in the near future.
机译:高密度电极网络(HDEN)展示了一种灵活的电穿孔程序,可用于在不同细胞类型中进行基因转染。在这项研究中,HDEN设备通过展示荧光蛋白不同类型的转基因的成功表达,证明了其在优化非病毒诱导鼠源性多能干细胞(iPSCs)效率方面的强大候选者的能力。已经观察到iPSCs基因表达的高而稳定的表达。还已经证明了在不同细胞类型中的合理的转染率和生存力。这意味着在不久的将来,结合质粒DNA诱导和HDEN的iPSCs产生的新替代方法可能是可行的。

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