• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>IEEE International Conference on Nanotechnology >A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood
【24h】

A novel dielectrophoresis-based microfluidic chip for antibody-free isolation of circulating tumor cells from blood

机译：一种基于介电电泳的新型微流控芯片，用于从血液中无抗体分离循环肿瘤细胞

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An antibody-free approach for high purity and high throughput isolation of circulating tumor cells (CTCs) from human blood in a microfluidic chip is presented. High throughput continuous CTC isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ~105 and a recovery rate of ~80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.
机译：提出了一种无抗体方法，可从微流控芯片中从人血中高纯度和高通量分离循环肿瘤细胞（CTC）。高通量连续CTC分离（> 1.2 ml / hr）通过垂直于连续通流的持续介电电泳（DEP）颗粒力实现。该设计允许根据交叉两边不同的临界DEP强度/迁移率的差异，将细胞连续分为不同的下游子通道。在1.2 ml / hr的高通量下，可实现〜105的富集因子和0.001％CTC样品的〜80％的回收率。与传统的物理方法（例如基于免疫磁学的方法和基于微流体生物标记物的方法）相比，所提出的无抗体芯片的分离纯度，回收率，通量和生存力表现出出色的性能，尤其是在高流量下的高富集方面速度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号