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Evaluation of rapid detection system for Escherichia coli in water samples

机译:水样大肠杆菌快速检测系统的评价

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We demonstrated a fast detection platform for Escherichia coli (E. coli) in water. The system is based on a fluorescence detection technique by determining enzyme β-D-glucuronidase (GUD) activity and relating that activity to E. coli concentration. We found the portable E. coli detection platform is able to detect both the fluorescence 4-MU signal from the reaction of GUD assay from 0.01-1 μg/ml and to enumerate the number of E. coli in the range of 105-107 CFU/ml. The required incubation time is in between 20-240 min. In addition, we can estimate the maximum of incubation time for detecting minimum of E. coli 1 CFU/ml from the equation to be 576 mins. Our preliminary study demonstrates that the system can be used to enumerate the number of E. coli in water on-site and requires less detection time than traditional E. coli methods.
机译:我们展示了一种用于水中大肠杆菌(E. coli)的快速检测平台。该系统基于荧光检测技术,该技术通过确定酶β-D-葡萄糖醛酸酶(GUD)活性并将该活性与大肠杆菌浓度相关联。我们发现便携式大肠杆菌检测平台既可以检测0.01-1μg/ ml的GUD检测反应产生的荧光4-MU信号,又可以列举105-107 CFU范围内的大肠杆菌数量/毫升。所需的孵育时间在20-240分钟之间。此外,我们可以从公式中估计出检测最小大肠杆菌1 CFU / ml所需的孵育时间的最大值为576分钟。我们的初步研究表明,该系统可用于枚举现场水中的大肠杆菌数量,并且比传统的大肠杆菌方法所需的检测时间更少。

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