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首页> 外文会议>World biomaterials congress >Repair of rotator cuff injury in rabbit animal model by in situ implantation of an injectable elastin-like recombinamer and allogeneic mesenchymal stem cells
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Repair of rotator cuff injury in rabbit animal model by in situ implantation of an injectable elastin-like recombinamer and allogeneic mesenchymal stem cells

机译：原位植入弹性蛋白样重组体和同种异体间充质干细胞修复兔动物肩袖损伤

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Introduction: One of the most common musculoskeletal pathologies is the shoulder pain, the rotator cuff injury affects more than 40% of patients over 60 years and causes disabling pain and loss of shoulder functionality. Despite the recent improvements and advances in the surgery, there remains a failure rate of between 20% and 70%. To obtain the tendon repair augmentation the new treatment strategies are directed to reinforce the mechanical properties and the biological healing capacity by implanting matrices, although the currently in use usually cause inflammatory reaction. To solve this problem, in this study we employed a modified version of an advanced matrix that has been demonstrating high efficiency and biocompatibility as bioactive support in regenerative medicine. It belongs to the family of (ELRs) the Elastin-like recombinamers with high mechanical performance designed as injectable thermogelling systems. The matrix was co-injected with rabbit bone marrow mesenchymal stem cells (rMSCs), under physiological conditions to evaluate the effect of cell therapy on the tendon pathology. Experimental Methods: ELRs were produced by DNA recombinant techniques and E. coli biosynthesis as described elsewhere. Rabbit mesenchymal stem cells (rMSCs) were obtained from rabbit bone marrow and BrdU stained. New Zealand rabbits with critical defect in the infraspinatus tendon were randomly allocated into 3 groups (negative control, treated with ELRs matrix, treated with ELRs matrix and rMSCs). At 10,1,4 and 16 weeks the animals were sacrificed and their tendons removed to perform biomechanical tests and immunohistochemical analysis. Results and Discussion: This study describes the use of injectable thermo-gelling scaffold whose applicability is focused specifically on regenerative medicine. The scaffold generated "in situ" under physiological condition was assayed in combination or not with rMSCs for the forming of an artificial matrix that improved tendon repair capacity. The results at 0,1,4 and 16 weeks were compared with which obtained adding ELR matrix and with the normal healing process the after injury of the infraspinatus tendon in rabbits. Figure 1. Showed the BRdU stained rMSCs and the ELR matrix after 1 month in the rabbit tendon implantation. In this work we observed a stronger and more physiological infraspinatus tendon repair using allogeneic rabbit mesenchymal stem cells in combination with an innovative ELRs thermogelling scaffold. Conclusion: The thermo-gelling property of the ELRs matrix has greatly facilitated the surgical technique as it is easier to handle (for injection), especially when used arthroscopically. The bioactivity and biocompatibility of the matrix enhanced the adhesion and proliferation of the rMSCs embedded in ELR-matrix in vitro. The in vivo tendon suture repair of the infraspinatus tendon has been studied in New Zealand rabbits. The rMSCs showed a high proliferation rate in the ELRs matrix when implanted in the tendons and a reinforced structural was obtained as was proved through the biomechanical, histological and immunohistochemical assays.
机译：简介：肩关节疼痛是最常见的肌肉骨骼疾病之一，60年来，肩袖损伤影响40％以上的患者，并导致残疾性疼痛和肩部功能丧失。尽管最近在外科手术方面有所改进和进步，但失败率仍然在20％至70％之间。为了获得增强的肌腱修复作用，新的治疗策略旨在通过植入基质来增强机械性能和生物学治愈能力，尽管目前使用的通常会引起炎症反应。为了解决这个问题，在这项研究中，我们采用了先进基质的改良版，该基质已经证明了高效和生物相容性作为再生医学中的生物活性支持物。它属于（ELR）类弹性蛋白重组蛋白，具有较高的机械性能，被设计为可注射的热胶凝体系。在生理条件下，将基质与兔骨髓间充质干细胞（rMSCs）共注射，以评估细胞疗法对肌腱病理的影响。实验方法：ELR通过DNA重组技术和大肠杆菌生物合成产生，如其他地方所述。从兔骨髓获得兔间充质干细胞（rMSCs），并对BrdU染色。将在睫状肌腱下有严重缺陷的新西兰兔随机分为3组（阴性对照组，用ELRs基质治疗，用ELRs基质和rMSCs治疗）。在10、1、4和16周时处死动物，并去除其肌腱以进行生物力学测试和免疫组织化学分析。结果与讨论：本研究描述了可注射热凝胶支架的使用，其适用性专门针对再生医学。将在生理条件下“原位”产生的支架与或不与rMSCs结合起来进行分析，以形成可改善肌腱修复能力的人工基质。将0、1、4和16周时的结果与兔ELP基质加损伤后的正常愈合过程进行比较。图1.显示了在兔肌腱植入物中1个月后，BRdU染色的rMSC和ELR基质。在这项工作中，我们观察到使用同种异体兔间充质干细胞与创新的ELRs热胶支架相结合，可以实现更强壮，更具生理性的肌下肌腱修复。结论：ELRs基质的热胶凝特性极大地促进了手术技术，因为它易于处理（用于注射），尤其是在关节镜下使用时。基质的生物活性和生物相容性增强了体外嵌入ELR基质的rMSC的粘附和增殖。在新西兰兔中，已研究了鼻下肌腱的体内肌腱缝合线修复。 rMSCs植入肌腱后在ELRs基质中显示出高增殖速率，并通过生物力学，组织学和免疫组织化学分析证明了其具有增强的结构。

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