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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Design of Comb-shaped Surface Electrode to Measure Signal from Tissue Cultured with Electric Stimulation
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Design of Comb-shaped Surface Electrode to Measure Signal from Tissue Cultured with Electric Stimulation

机译：梳形表面电极设计用于测量电刺激组织的信号

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The comb-shaped surface electrode has been designed to measure the signal from the tissue cultured with electric stimulation. The pattern of the thin film of the surface electrode was made of indium tin oxide (ITO) coated on the glass by the photolithography technique. C2C12 (mouse myoblast cell line) was cultured for 13 days on the patterned scaffold. The electric pulses (period 1 s, amplitude 10 V, duration 0.002 s) were applied for 10 minutes per day. The experimental results show that myoblasts make orientation along the comb-shaped surface electrode, which can be observed through the transparent surface electrode by the optical microscope. The change of the electric resistance of the cultured tissue is able to be detected between electrodes of the experimental system in vitro.
机译：梳形表面电极已设计为测量来自在电刺激下培养的组织的信号。表面电极的薄膜的图案由通过光刻技术涂覆在玻璃上的铟锡氧化物（ITO）制成。在图案化的支架上将C2C12（小鼠成肌细胞细胞系）培养13天。每天施加10分钟的电脉冲（周期1 s，幅度10 V，持续时间0.002 s）。实验结果表明，成肌细胞沿着梳状表面电极定向，这可以通过光学显微镜通过透明表面电极观察到。能够在体外在实验系统的电极之间检测培养的组织的电阻的变化。

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