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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Effect of Shear Stress in Flow on Cultured Cell: Using Rotating Disk at Microscope
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Effect of Shear Stress in Flow on Cultured Cell: Using Rotating Disk at Microscope

机译：流动中的剪切应力对培养细胞的影响：在显微镜下使用转盘

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An experimental system of the Couette type flow with a rotating disk has been designed to apply wall shear stress quantitatively on the cell culture at the microscopic observation in vitro. The shear stress on the wall is calculated with an estimated Couette type of the velocity profile between the rotating disk and the culture plate. The constant rotational speed (lower than 400 rpm) produces the wall shear stress lower than 2 Pa. The rotating disk system is mounted on the stage of an inverted phase contrast microscope to observe the behavior of cells adhered on the plate under the shear flow. Two kinds of cells were used in the test: C2C12 (mouse myoblast cell line), and MC3T3-E1 (mouse osteoblast precursor cell line). The experiments show that C2C12 tends to make orientation diagonal to the stream line, and that MC3T3-E1 tends to make orientation to the stream line. Deformation and exfoliation of cells can be observed under controlled wall shear stress by the experimental system.
机译：已经设计了具有旋转盘的库埃特（Couette）型流动的实验系统，以便在体外显微镜观察下将壁切应力定量地施加到细胞培养物中。壁上的剪切应力是用旋转盘和培养板之间的速度曲线的估算库埃特（Couette）类型计算得出的。恒定转速（低于400 rpm）产生的壁切应力低于2 Pa。将转盘系统安装在反相显微镜的平台上，以观察剪切流下粘附在板上的细胞的行为。测试中使用了两种细胞：C2C12（小鼠成肌细胞细胞系）和MC3T3-E1（小鼠成骨细胞前体细胞系）。实验表明，C2C12倾向于使方向垂直于流线，而MC3T3-E1倾向于使方向垂直于流线。实验系统可在受控的壁切应力作用下观察到细胞的变形和剥落。

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