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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Behavior of Cells in Excess Gravitational Field: Using Centrifuge
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Behavior of Cells in Excess Gravitational Field: Using Centrifuge

机译：重力场中细胞的行为：使用离心机

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摘要

The effect of mechanical field on orientation and extension of cells has been studied using centrifuge in vitro. Four kind of cells were used in the test: C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse), L929 (fibroblast connective tissue of C3H mouse), HUVEC (Human Umbilical Vein Endothelial Cells), Neuro-2a (a mouse neural crest-derived cell line). To apply the mechanical force field for 24 hours to the cells adhered on the glass plate, the plate was set in the tube in a conventional centrifugal machine, which was placed in an incubator. Variation was made at the angular position of the plate in the tube to make variation at the direction of the force on the surface of scaffold: normal and tangential. The experiment shows following results. The elongation of neurites of Neuro2a is accelerated in the excess gravitational force field. L929 tends to tilt to the direction of the tangential force. C2C12 tends to make orientation at diagonal direction of the tangential force. The cell tends to extend the area of adhesion at the excess gravitational force field. C2C12 tends to change the shape at the excess gravitational field.
机译：体外使用离心机研究了机械场对细胞定向和延伸的影响。测试中使用了四种细胞：C2C12（源自C3H小鼠的横纹肌的小鼠成肌细胞系），L929（C3H小鼠的成纤维细胞结缔组织），HUVEC（人脐静脉内皮细胞），Neuro-2a（小鼠神经c来源的细胞系）。为了对粘附在玻璃板上的细胞施加24小时的机械力场，将板置于常规离心机中的试管中，该离心机置于培养箱中。在管中板的角位置进行了更改，以使在脚手架表面上的力的方向进行更改：法向和切向。实验显示了以下结果。 Neuro2a神经突的伸长在多余的重力场中被加速。 L929倾向于向切向力的方向倾斜。 C 2 C 12倾向于在切向力的对角线方向上取向。电池趋向于在过大的重力场处扩展粘附区域。 C 2 C 12倾向于在过大的引力场处改变形状。

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