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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Effect of Aspect Ratio of Checkered (Ichimatsu) Convexo-concave Micro-pattern on Orientation of Cultured Cells
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Effect of Aspect Ratio of Checkered (Ichimatsu) Convexo-concave Micro-pattern on Orientation of Cultured Cells

机译：方格（市松）凹凸微图案的长宽比对培养细胞方向的影响

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摘要

The effect of the pattern of micro ridges on orientation of the cell culture has been studied in vitro. The checkered (Ichimatsu) convexo-concave pattern has been designed with micro quadrangular prisms in the square area of 1 mm × 1 mm on a disk of glass for a scaffold by the lithography technique. Each prism has the following dimension: 0.01 mm length, and 0.005 mm (or 0.001 mm) height. Variation has been made on the width of the prism: 0.005 mm, 0.008 mm, and 0.01 mm. The variation of the width makes the variation on the aspect ratio of the top rectangular surface of the prism: 1, 1.25, and 2. Three kinds of cells were used in the test: C2C12 (mouse myoblast cell line), Neuro-2a (a mouse neural crest-derived cell line), and Hepal-6 (mouse hepatoma cell line). Cells were seeded on the micro pattern, and incubated for 21 days in the Dulbecco's Modified Eagle Medium containing 10% fetal bovine serum and 1% penicillin/ streptomycin. The cells were observed with a phase contrast microscope. The experimental results show that the orientation of myotubes can be controlled by the aspect ratio of the checkered micro convexo-concave pattern of the surface of the scaffold.
机译：体外研究了微脊的图案对细胞培养方向的影响。方格（一松）凸凹图案已通过光刻技术在支架用玻璃盘上以1 mm×1 mm的正方形面积设计有微型四边形棱镜。每个棱镜的尺寸如下：长度为0.01毫米，高度为0.005毫米（或0.001毫米）。棱镜的宽度已发生变化：0.005 mm，0.008 mm和0.01 mm。宽度的变化使棱镜的顶部矩形表面的长宽比发生变化：1、1.25和2。测试中使用了三种细胞：C2C12（小鼠成肌细胞系），Neuro-2a（小鼠神经c衍生的细胞系）和Hepal-6（小鼠肝癌细胞系）。将细胞接种在微图案上，并在含有10％胎牛血清和1％青霉素/链霉素的Dulbecco改良Eagle培养基中孵育21天。用相差显微镜观察细胞。实验结果表明，可以通过支架表面的方格微凹凸图案的长宽比来控制肌管的方向。

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