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首页> 外文会议>World multi-conference on systemics, cybernetics and informatics >Design of Slit between Micro Cylindrical Pillars for Cell Sorting
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Design of Slit between Micro Cylindrical Pillars for Cell Sorting

机译：微圆柱柱间缝隙的细胞分选设计

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Micro slits have been designed between micro cylindrical pillars to sort biological cells. Micro cylindrical pillars of 0.02 mm diameter and 0.055 mm height were fabricated on the glass plate using the photolithography technique. Variation was made on the gap between pillars: 0.01 mm, 0.02 mm, 0.03 mm, and 0.04 mm. The micro pillars are set in the flow path between parallel plates, of which dimension of the cross section has 5 mm width and 0.055 mm height. Three types of biological cells were used in the test alternatively: C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse), Hepal-6 (mouse hepatoma cell line of C57L mouse), or swine red blood cell. The suspension of cells was introduced into the slits by the syringe pump at the flow rate between 0.4 and 500 cm~3/hour. The deformation of the cell at the slit can be observed by the microscope. The experimental results show that the designed slit has capability for sorting cells according to the size and defonnability of the cell.
机译：在微型圆柱体支柱之间设计了微缝隙，以对生物细胞进行分类。使用光刻技术在玻璃板上制作了直径为0.02 mm，高度为0.055 mm的微型圆柱体。支柱之间的间隙发生了变化：0.01 mm，0.02 mm，0.03 mm和0.04 mm。微型支柱设置在平行板之间的流路中，其横截面尺寸为宽度为5毫米，高度为0.055毫米。备选地，在测试中使用了三种类型的生物细胞：C2C12（源自C3H小鼠的横纹肌的小鼠成肌细胞细胞系），Hepal-6（C57L小鼠的小鼠肝癌细胞系）或猪红细胞。通过注射泵将细胞悬浮液以0.4至500cm 3 /小时之间的流速引入狭缝中。狭缝处的细胞变形可以通过显微镜观察。实验结果表明，所设计的狭缝具有根据细胞的大小和可修饰性对细胞进行分选的能力。

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