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首页> 外文会议>International astronautical congress >ACCELERATION OF CRITICAL BONE DEFECT HEALING BY ULTRASOUND TREATMENT IN A OSTEOPENIA MODEL
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ACCELERATION OF CRITICAL BONE DEFECT HEALING BY ULTRASOUND TREATMENT IN A OSTEOPENIA MODEL

机译：骨质疏松模型中超声治疗加速关键性骨缺损愈合

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Bone defects, usually caused by trauma, inflammation and tumors, may lead to loss of limb functions and substantially affect the quality of human life. When bone is impaired, it is essential to find effective methods to accelerate bone healing. Low-intensity ultrasound (LIUS), which is a unique form of mechanical energy, can transmit through and into living tissues as dynamic acoustic radiation force in the local region. The aim of this study was to evaluate the effect of LIUS induced healing in a rat tibial critical defect. A total of 40 skeletally mature female Lewis rats were used in this study. Bilaterally, the surgical bone defects of 2-3 mm in diameter were made at proximal tibia. Subsequently, the defects of the left tibia (LIUS group) received daily treatment of low-intensity pulsed ultrasound. A modified repetitive frequency at 100 Hz and an ultrasound characteristic frequency of 1 MHz and a pulse width of 200 s at an intensity of 30 mW/cm2 were used for the stimulation for 20 minutes/day. After 2 weeks, the rats were euthanized and the tibias were assessed using micro-CT scanning, histology and mechanical testing. Trabecular bone volume fraction (BV/TV), connectivity density (Conn.D), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), trabecular number (Tb.N), and tissue mineral density (mgHA/cm3) were evaluated. Osteomcasure software was used to evaluate the newly formed trabecular bone in the defections of tibia. Four-point bending was used for examining the stiffness and strength using a MTS mechanical testing system. In comparison to control group, LIUS treated tibia showed increased in BV/TV, Conn.D, Tb.N, and BMD, and significant decrease in Tb.Sp. Osteomeasure analysis showed a similar trend, which is in line with the micro-CT results. BV/TV and Tb.Th were higher in LIPUS group than those in control group, and Tb.Sp was lower in LIUS group than that in control group. Ultimate load (N) at mid-shaft of tibia showed an increasing trend, but no significant increase was observed following the exposure to LIPUS, when compared to control group (p=0.056). The histological analysis and μCT data revealed that new bone formed in both LIUS therapy and control with tibia defect; and LIUS therapy can significantly increase new trabecular bone volume. Moreover, bone stiffness and ultimate load of LIUS treated bone were higher than no-therapy group. Two weeks seem to be the key period for bone regeneration after bone defect surgery. Performing LIUS on bone defect could efficiently accelerate bone healing.
机译：通常由外伤，炎症和肿瘤引起的骨骼缺陷可能导致肢体功能丧失，并严重影响人类生活质量。当骨骼受损时，必须找到有效的方法来加速骨骼愈合。低强度超声（LIUS）是机械能的一种独特形式，它可以作为动态声辐射力在局部区域传播通过并进入活组织。这项研究的目的是评估LIUS诱导的大鼠胫骨严重缺损愈合的效果。这项研究共使用了40只骨骼成熟的雌性Lewis大鼠。双侧在胫骨近端进行直径为2-3 mm的手术骨缺损。随后，左胫骨缺损（LIUS组）每天接受低强度脉冲超声治疗。以100 Hz的修改重复频率和1 MHz的超声特征频率以及在30 mW / cm2的强度下200 s的脉冲宽度用于刺激20分钟/天。 2周后，对大鼠实施安乐死，并使用微型CT扫描，组织学和力学测试评估胫骨。骨小梁体积分数（BV / TV），连接密度（Conn.D），骨小梁厚度（Tb.Th），骨小梁分离度（Tb.Sp），骨小梁数（Tb.N）和组织矿物质密度（mgHA / cm3 ）进行了评估。 Osteomcasure软件用于评估胫骨畸形中新形成的小梁骨。使用MTS机械测试系统，使用四点弯曲检查硬度和强度。与对照组相比，LIUS治疗的胫骨的BV / TV，Conn.D，Tb.N和BMD升高，而Tb.Sp显着降低。骨测量分析显示了相似的趋势，这与微CT结果相符。 LIPUS组的BV / TV和Tb.Th高于对照组，LIUS组的Tb.Sp低于对照组。与对照组相比，胫骨中轴的极限负荷（N）呈增加趋势，但暴露于LIPUS后未观察到明显增加（p = 0.056）。组织学分析和μCT数据显示，LIUS治疗和对照中均形成了新骨并伴有胫骨缺损。和LIUS疗法可以显着增加新的小梁骨体积。而且，LIUS治疗的骨的骨刚度和最终负荷高于未治疗组。骨缺损手术后两周似乎是骨再生的关键时期。对骨缺损进行LIUS可以有效地促进骨愈合。

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